PMID: 11333601May 3, 2001Paper

Contribution to the early diagnosis of bacteremia: microbial growth detection in liquid culture media by ultrasound

Enfermedades infecciosas y microbiología clínica
J R Maestre, F R Montero de Espinosa

Abstract

Nosocomial infection is an important problem because the number of patients daily affected in big hospitals. A big effort exists to develop techniques able to early detect the micro-organisms which cause the infection. The ultrasound is a mechanical radiology technique widely used in Medicine for diagnosis and therapy. It is also well known that this radiation can be used to control relative changes of several physico-chemical parameters in liquids. As an example, the velocity an attenuation of acoustic waves coming through a liquid can be accurately measured. The developed technique consists of an ultrasonic chamber immersed into a thermostatized water bath with two transducers operating in through-transmission. Different culture bottles were placed in between the transducers to live the ultrasound to come across the sample. Several micro-organisms with controlled concentrations, chosen between the most common in sepsis clinical, were used to inoculate each bottle. In the case of aerobic metabolism, the carbon dioxide gas produced by bacteria introduce elastic changes into the liquid which modify both the propagation velocity and the attenuation of the ultrasound. The continuous monitoring of the time-of-flight and the amplitu...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.