Contributions of an endonuclease IV homologue to DNA repair in the African swine fever virus

Biochemistry
Brandon J Lamarche, Ming-Daw Tsai

Abstract

We recently demonstrated that African swine fever virus DNA polymerase X (Pol X) is extremely error-prone during single-nucleotide gap-filling and that the downstream ASFV DNA ligase seals 3' mismatched nicks with high efficiency. To further assess the credence of our hypothesis that these proteins may promote viral diversification by functioning within the context of an aberrant DNA repair pathway, herein we characterize the third protein expected to function in this system, a putative AP endonuclease (APE). Assays of the purified protein using oligonucleotide substrates unequivocally establish canonical APE activity, 3'-phosphatase and 3'-phosphodiesterase activities (in the context of a single-nucleotide gap), 3' --> 5' exonuclease activity (in the context of a nick), and nucleotide incision repair activity against 5,6-dihydrothymine. The 3' --> 5' exonuclease activity is shown to be highly dependent upon the identity of the nascent 3' base pair and to be inhibited when 2-deoxyribose-5-phosphate, rather than phosphate, constitutes the 5' moiety of the nick. ASFV APE retains activity when assayed in the presence of EDTA but is inactivated by incubation with 1,10-phenanthroline in the absence of a substrate, suggesting that it...Continue Reading

References

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Jun 9, 2005·Biochemistry·Brandon J LamarcheMing-Daw Tsai

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Citations

Nov 13, 2012·Virus Research·Linda K DixonChris Upton
Nov 5, 2013·Virus Research·Modesto Redrejo-Rodríguez, María L Salas
Jul 14, 2007·DNA Repair·Viswanath BandaruSusan S Wallace
Oct 29, 2008·Journal of Molecular Biology·Benedetta A Sampoli BenítezTamar Schlick
Aug 19, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Solenne CostardLinda K Dixon
Mar 21, 2020·Cell Discovery·Yiqing ChenJianhua Gan
Mar 18, 2021·Cell Discovery·Yiqing ChenJianhua Gan
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiwon ChoiSoon B Hwang

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