Contributions of capsule, lipoproteins and duration of colonisation towards the protective immunity of prior Streptococcus pneumoniae nasopharyngeal colonisation.

Vaccine
Jonathan M CohenJeremy S Brown

Abstract

Live attenuated vaccines have been proposed as a strategy to induce protective immunity against infectious diseases. Recent data have demonstrated that nasopharyngeal colonisation with Streptococcus pneumoniae induces protective immunity against subsequent invasive infection, suggesting nasal vaccination with live attenuated bacteria could be a preventative strategy. However the bacterial factors affecting the strength of this adaptive immune response remain unclear. In a direct comparison with the parent wild-type strain, we found that colonisation with bacteria lacking either capsule or surface lipoproteins led to significantly diminished protection. Immunity after colonisation was not dependent on serum IgG to capsular antigens. Colonisation density and duration was reduced for all the non-protective strains, suggesting that protective immunity maybe related to the extent of nasopharyngeal bacterial exposure. To investigate this hypothesis, we utilised an auxotrophic bacterial Δpab strain where duration of colonisation could be controlled by supply and removal of para-amino-benzoic acid (PABA) to mouse drinking water. Supporting colonisation with the Δpab strain for 5 days with PABA led to a faster serum antibody response co...Continue Reading

References

Feb 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Richard MalleyDouglas T Golenbock
Mar 5, 2004·The Lancet Infectious Diseases·D BogaertP W M Hermans
Jul 5, 2005·The Journal of Infectious Diseases·David GoldblattElizabeth Miller
Sep 12, 2007·PLoS Pathogens·Jose YusteJeremy S Brown
Feb 22, 2008·Current Medicinal Chemistry·Peter M Moyle, Istvan Toth
Jun 11, 2009·The Journal of Clinical Investigation·Zhe ZhangJeffrey N Weiser
Nov 3, 2009·Lancet·Tom van der Poll, Steven M Opal
Jan 6, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Richard Malley
Nov 19, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S ShomaA van Belkum
Feb 16, 2011·Cell Host & Microbe·Kristin L MoffittRichard Malley

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Citations

Mar 1, 2016·Frontiers in Microbiology·Shahper N Khan, Asad U Khan
Jan 27, 2019·Applied and Environmental Microbiology·Sudhanshu ShekharFernanda Cristina Petersen
Mar 5, 2021·Frontiers in Cellular and Infection Microbiology·Murtadha Q AliSven Hammerschmidt

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Methods Mentioned

BETA
PCR
lavage
ELISAs
flow cytometry
ELISA

Software Mentioned

Plex Manager
Bio

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