Contributions of Dickkopf-1 to Obesity-Induced Bone Loss and Marrow Adiposity

JBMR Plus
Juliane ColditzMartina Rauner

Abstract

Low bone strength in overweight individuals is a significant medical problem. One important determinant of mesenchymal stem cell fate into osteoblasts or adipocytes is the Wnt signaling pathway. We recently showed that Dickkopf-1 (DKK1), a potent Wnt inhibitor, is upregulated in obese mice. In this study, we investigated the role of DKK1 in the pathogenesis of obesity-induced bone loss using global and tissue-specific KO mice. Obesity was induced in 8-week-old male mice with an inducible global (Rosa26-CreERT2) or osteoprogenitor- (Osx-Cre-) specific deletion of Dkk1 with a high-fat diet (HFD) containing 60% fat. After 12 weeks, body weight, bone volume, bone fat mass, and bone turnover were assessed. Dkk1 fl/fl ;Rosa26-CreERT2 mice experienced a similar increase in body weight and white fat pads as control mice. A HFD significantly reduced trabecular bone mass and the bone formation rate in Cre- mice and Dkk1 fl/fl ;Rosa26-CreERT2 mice. Interestingly, Dkk1 fl/fl ;Rosa26-CreERT2 mice were protected from HFD-induced cortical bone loss. Furthermore, a HFD was associated with increased bone marrow fat in the femur, which was less pronounced in Dkk1 fl/fl ;Rosa26-CreERT2 mice. Mice with an osteoprogenitor-specific Dkk1 deletion sho...Continue Reading

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Citations

Oct 20, 2020·Frontiers in Endocrinology·Nathalie S AlekosRyan C Riddle

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Methods Mentioned

BETA
PCR
ELISAs
ELISA
transgenic

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