PMID: 7520094Sep 1, 1994Paper

Contributions of DNA polymerase subdomains to the RNase H activity of human immunodeficiency virus type 1 reverse transcriptase

Journal of Virology
J S SmithM J Roth

Abstract

Previous studies showed that an isolated human immunodeficiency virus type 1 (HIV-1) RNase H domain expressed as a fusion protein is highly active in Mn2+, but activity was dependent on a hexahistidine tag located at either the carboxyl or amino terminus of the fusion protein (J. Smith and M. Roth, J. Virol. 67:4037-4049, 1993). It was postulated that a histidine tag can somehow provide a function normally associated with the DNA polymerase domain of HIV-1 reverse transcriptase. To determine the contributions of the DNA polymerase subdomains of HIV-1 reverse transcriptase to its RNase H activity, we have characterized the activity of isolated RNase H domains which include either portions of the connection, the entire connection, or both the thumb and connection as N-terminal extensions. Including increasing lengths of these domains at the N terminus of the RNase H resulted in a progressive increase in Mn(2+)-dependent RNase H activity that was independent of a histidine tag. Activity of the isolated RNase H domains was also stimulated by the addition of independently purified polymerase subdomains. Further, this stimulation was shown to be a result of direct physical interactions between the thumb, connection, and RNase H domai...Continue Reading

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Citations

Apr 25, 1997·The Journal of Biological Chemistry·C PalaniappanR A Bambara
Aug 25, 2000·The Journal of Biological Chemistry·M WisniewskiR A Bambara
Aug 17, 2006·Journal of Virology·David LimStephen P Goff
Jul 20, 2011·Antimicrobial Agents and Chemotherapy·Richard V FariasOscar Leon
Apr 1, 2010·Viruses·Greg L Beilhartz, Matthias Götte
May 7, 2014·Antimicrobial Agents and Chemotherapy·Angela CoronaEnzo Tramontano
Feb 21, 2009·The FEBS Journal·James J Champoux, Sharon J Schultz
May 23, 2002·The Journal of Biological Chemistry·Michele WisniewskiRobert A Bambara
Apr 2, 2010·Cellular and Molecular Life Sciences : CMLS·Alon Herschhorn, Amnon Hizi
Jan 22, 2017·Nucleic Acids Research·Malgorzata FigielMarcin Nowotny
Apr 13, 2007·Journal of Virology·Krista A Delviks-FrankenberryVinay K Pathak
Jul 7, 2017·The Journal of Biological Chemistry·Aparna KishorGerald M Wilson
Feb 14, 2012·Retrovirology·Berit LeoBirgitta M Wöhrl
Feb 12, 2008·Virus Research·Sharon J Schultz, James J Champoux

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