Contributions of individual σB-dependent general stress genes to oxidative stress resistance of Bacillus subtilis.

Journal of Bacteriology
Alexander RederM Hecker

Abstract

The general stress regulon of Bacillus subtilis comprises approximately 200 genes and is under the control of the alternative sigma factor σ(B). The activation of σ(B) occurs in response to multiple physical stress stimuli as well as energy starvation conditions. The expression of the general stress proteins provides growing and stationary nonsporulating vegetative cells with nonspecific and broad stress resistance. A previous comprehensive phenotype screening analysis of 94 general stress gene mutants in response to severe growth-inhibiting stress stimuli, including ethanol, NaCl, heat, and cold, indicated that secondary oxidative stress may be a common component of severe physical stress. Here we tested the individual contributions of the same set of 94 mutants to the development of resistance against exposure to the superoxide-generating agent paraquat and hydrogen peroxide (H(2)O(2)). In fact, 62 mutants displayed significantly decreased survival rates in response to paraquat and/or H(2)O(2) stress compared to the wild type at a confidence level of an α value of ≤ 0.01. Thus, we were able to assign 47 general stress genes to survival against superoxide, 6 genes to protection from H(2)O(2) stress, and 9 genes to the survival...Continue Reading

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Citations

Oct 16, 2012·Applied and Environmental Microbiology·Heidy M W den BestenTjakko Abee
Feb 2, 2013·PloS One·Philippe LanotteChristo Atanassov
Jan 25, 2017·Biomolecules·Juan Campos GuillenVerónica Morales Tlalpan
Jun 29, 2014·Applied and Environmental Microbiology·Fernando Santos-EscobarMario Pedraza-Reyes
Feb 12, 2020·Integrative and Comparative Biology·Carla Y Bonilla
Feb 27, 2015·Biological Chemistry·Melanie Hillion, Haike Antelmann
Jul 21, 2015·Photochemistry and Photobiology·Jeroen B van der Steen, Klaas J Hellingwerf
Apr 22, 2020·Nature Communications·Jesús Cámara-AlmirónDiego Romero
May 16, 2017·Biotechnology for Biofuels·Gumpanat MahipantAlisa S Vangnai
Feb 6, 2021·World Journal of Microbiology & Biotechnology·Hoai T Tran, Carla Y Bonilla
Feb 13, 2021·Current Opinion in Microbiology·Jeanine RismondoAngelika Gründling
Nov 28, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Bimal PrajapatiJan Maarten van Dijl

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