Contributions of Rho-kinase and AMP-related kinase signaling pathways to responses mediated by endothelium-derived contracting factors in diabetic rat aorta

Canadian Journal of Physiology and Pharmacology
Cennet BalcilarV Melih Altan

Abstract

Diabetes-induced endothelial damage leads to vascular dysfunction. The current study investigated the effects of short-term (4-week) streptozotocin (STZ)-induced diabetes on responses mediated by endothelium-derived contracting factors (EDCFs) as well as possible contributions of Rho-kinase and AMP-activated kinase (AMPK) signaling pathways. The effects of STZ-diabetes on vascular function were examined in isolated thoracic aorta preparations of 30-week-old rats (n = 27). The diabetes-associated changes in vascular function were studied with calcium ionophore A23187, acetylcholine, Rho-kinase inhibitor Y27632 ((R)-(+)-trans-4-(1-aminoethyl)-N-(4-pyridyl)cyclohexanecarboxamide dihydrochloride), and AMPK activator AICAR (5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside). The phosphorylation of acetyl-CoA carboxylase, AMPK, and phospholamban and the protein levels of sarcoplasmic/endoplasmic Ca2+-ATPase 2 (SERCA2) and Rho-associated protein kinase (ROCKII) were measured in aortic preparations. Although the acetylcholine-mediated relaxation responses were preserved in 4-week STZ-induced diabetes, the increased activation of the Rho-kinase pathway was demonstrated via twofold enhancement in A23187-mediated contractile responses a...Continue Reading

References

Jul 1, 1993·The American Journal of Physiology·B E RobertsonM T Nelson
Jan 19, 2005·British Journal of Pharmacology·Paul M VanhoutteStefano Taddei
May 10, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Yingbi ZhouJay L Zweier
Sep 15, 2005·British Journal of Pharmacology·Pascale GluaisMichel Feletou
Jun 27, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Pascale GluaisMichel Félétou
Apr 21, 2007·The Journal of Physiology·Françoise GoirandAnne Garnier
Feb 23, 2008·Circulation Research·Sven T PlegerPatrick Most
Apr 23, 2008·The Journal of Biological Chemistry·Sandrine HormanMark H Rider
Feb 6, 2009·The Journal of Pharmacology and Experimental Therapeutics·Calvin K Y ChanPaul M Vanhoutte
Mar 17, 2009·Pharmacology & Therapeutics·Eva H C Tang, Paul M Vanhoutte
Nov 17, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Daisuke Nagata, Yasunobu Hirata
Feb 16, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Steven G DennissJames W E Rush
May 13, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Lahouaria HadriRoger J Hajjar
Oct 26, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Rebecca J Ford, James W E Rush
Dec 15, 2010·Journal of Clinical Pharmacology·Marlies BosselaarCees J Tack
Aug 20, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Marion GayardMalvyne Rolli-Derkinderen
Sep 6, 2012·Journal of Vascular Research·Anneloes MartinsenNicole Morel
Mar 28, 2013·Cardiovascular Diabetology·Figen Amber CicekBelma Turan
May 4, 2013·European Heart Journal·Francesco PaneniFrancesco Cosentino
Jun 27, 2014·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Xia CaoZhiyi Tang

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.