PMID: 8609613Feb 16, 1996Paper

Contributions of supercoiling to Tn3 resolvase and phage Mu Gin site-specific recombination

Journal of Molecular Biology
K R BenjaminN R Cozzarelli

Abstract

Members of the resolvase/invertase family of site-specific recombinases require supercoiled substrates containing two recombination sites. To dissect the roles of supercoiling in recombination by the Tn3 and gamma delta resolvases and the phage Mu Gin invertase, we used substrates that provided some but not all of the topological features of the standard substrate. We divided the Tn3 resolvase reaction into two stages, synapsis and postsynapsis. Using structural and functional topological analyses, we verified that the resolvase synaptic complexes with nicked catenanes were recombination intermediates. The requirement for supercoiling was even less stringent for the gamma delta resolvase, which recombined nicked catenanes about half as well as it did supercoiled substrates. Gin recombination of catenanes occurred even if the recombinational enhancer was on a nicked ring, as long as both crossover sites were on a supercoiled ring. Therefore, supercoiling is required at the Gin crossover sites but not at the enhancer. We conclude that solely conformational effects of supercoiling are required for resolvase synapsis and the function of the Gin enhancer, but that a torsional effect, probably double helix unwinding, is needed for Tn...Continue Reading

Citations

Dec 15, 2010·Molecular Microbiology·Betty M BookerN Patrick Higgins
Jul 9, 2016·Nucleic Acids Research·Alexandra PokhilkoSean D Colloms
Dec 14, 1999·Molecular Microbiology·R SchneiderG Muskhelishvili
Jul 6, 2004·Archives of Microbiology·Bae Hoon KimYong Keun Park
Apr 28, 2005·Molecular Microbiology·Richard A SteinN Patrick Higgins
Jul 18, 1997·The Journal of Biological Chemistry·H M LimM Nadal
Feb 26, 2009·Nucleic Acids Research·Zhirong LiuLynn Zechiedrich
Oct 22, 1998·Molecular Microbiology·P Staczek, N P Higgins
Dec 15, 2000·Journal of Bacteriology·O Z Nanassy, K T Hughes
May 17, 2017·Biophysics Reviews·Georgi Muskhelishvili, Andrew Travers
Aug 29, 1997·Journal of Molecular Biology·Z Wang, P Dröge
Jun 17, 1999·Journal of Molecular Biology·N J CrisonaN R Cozzarelli
Jun 17, 1999·Journal of Molecular Biology·J BathS D Colloms
Mar 17, 1999·Methods : a Companion to Methods in Enzymology·R R SindenP R Kramer
Mar 30, 2001·Biochimie·A TraversG Muskhelishvili
Jun 25, 2015·Microbiology Spectrum·N Patrick Higgins, Alexander V Vologodskii
Sep 30, 1998·Cell·B J PeterN R Cozzarelli
Mar 21, 1997·Cell·S D CollomsD J Sherratt

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.