Contributions of the mitogen-activated protein (MAP) kinase backbone and phosphorylation loop to MEK specificity.

The Journal of Biological Chemistry
M J RobinsonM H Cobb

Abstract

To examine the specificity of MEKs for MAP kinase family members, we determined the abilities of several MEK isoforms to phosphorylate mutants of the MAP kinase ERK2 and the related kinase ERK3 which are modified in the phosphorylation loop. The ERK2 mutants included mutations of the two phosphorylation sites, mutations of the acidic residue between these two sites, and mutations that shorten the length of this loop. All mutants were tested for phosphorylation by six mammalian MEKs and compared with several wild type MAP kinases. MEK1 and MEK2 phosphorylate a majority of the ERK2 mutants. MEK2 but not MEK1 will phosphorylate ERK3. Alteration of the residue between the two phosphorylation sites neither dramatically affected the activity of MEK1 and MEK2 toward ERK2 nor conferred recognition by other MEKs. Likewise, reduction of the length of the phosphorylation loop only partially reduces recognition by MEK1 and MEK2 but does not promote recognition by other MEKs. Thus other yet to be identified factors must contribute to the specificity of MEK recognition of MAP kinases.

References

Dec 1, 1995·The Journal of Biological Chemistry·J M EnglishM H Cobb
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·S XuM H Cobb
May 26, 1995·The Journal of Biological Chemistry·G ZhouJ E Dixon
Jun 23, 1995·The Journal of Biological Chemistry·M H Cobb, E J Goldsmith
Nov 1, 1994·Trends in Biochemical Sciences·R J Davis
Feb 24, 1994·Nature·F ZhangE J Goldsmith
May 12, 1994·Nature·J M KyriakisJ R Woodgett
Apr 1, 1993·Trends in Biochemical Sciences·E Nishida, Y Gotoh
Apr 12, 1996·The Journal of Biological Chemistry·M ChengM H Cobb
Feb 9, 1996·The Journal of Biological Chemistry·J HanR J Ulevitch
Feb 23, 1996·The Journal of Biological Chemistry·E R Butch, K L Guan
Jun 7, 1996·The Journal of Biological Chemistry·T MoriguchiM Hagiwara
Dec 6, 1996·Science·R W KingM W Kirschner

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Citations

May 1, 2009·Pflügers Archiv : European journal of physiology·Dimitar P ZankovMinoru Horie
Dec 4, 2001·Biology of the Cell·H Enslen, R J Davis
Apr 1, 1997·Current Opinion in Cell Biology·M J Robinson, M H Cobb
Jun 15, 1999·Trends in Biochemical Sciences·T R Soderling
Apr 14, 2009·Journal of the American Chemical Society·Adrian Gustavo TurjanskiJ Silvio Gutkind
Jan 3, 2001·The Plant Journal : for Cell and Molecular Biology·K Mockaitis, S H Howell
Feb 15, 2011·The Journal of Biological Chemistry·Alina De la Mota-PeynadoAlexander Beeser
Feb 7, 2009·The Journal of Biological Chemistry·A Jane BardwellLee Bardwell
Jun 20, 1998·The EMBO Journal·J M JacquéM Stevenson
May 14, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sangwook JungNicholas P Poolos
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·Z WangE J Goldsmith
Jan 3, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Teodor-D BrumeanuSofia Casares
May 14, 2010·The Journal of General Virology·Terence N BukongJean-Marc Jacqué
Dec 13, 2006·Biochimica Et Biophysica Acta·Phillipe Coulombe, Sylvain Meloche
Sep 23, 1997·Cell·B J CanagarajahE J Goldsmith
Feb 4, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Ji-an HuJinhua Fang
Mar 3, 2017·Scientific Reports·Maria PeranderOle-Morten Seternes
Jun 8, 1999·The Journal of Biological Chemistry·J L WilsbacherM H Cobb
Oct 21, 2006·Biochemical Society Transactions·L Bardwell
Sep 2, 2000·The Journal of Biological Chemistry·M KarandikarM H Cobb
Oct 24, 1998·The Journal of Biological Chemistry·M HutchisonM H Cobb
Jan 19, 2002·The Journal of Biological Chemistry·Terence P HerbertChristopher G Proud
Feb 2, 2002·The Journal of Biological Chemistry·Fred L RobinsonMelanie H Cobb
Dec 16, 1998·The Journal of Biological Chemistry·J W RamosM H Ginsberg
Apr 25, 2019·International Journal of Molecular Sciences·Jung-Min KimJae-Hyuck Shim
Apr 22, 2019·The Journal of Chemical Physics·Mikita Misiura, Anatoly B Kolomeisky
Aug 10, 2017·Oxidative Medicine and Cellular Longevity·Saud AlarifiRafa S Almeer
Jul 20, 2019·Biochemical and Biophysical Research Communications·Alexander A TokmakovKen-Ichi Sato

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