Contributions of XylR CcpA and cre to diauxic growth of Bacillus megaterium and to xylose isomerase expression in the presence of glucose and xylose

Molecular & General Genetics : MGG
D Schmiedel, W Hillen

Abstract

Bacillus megaterium shows diauxic growth in minimal medium containing glucose and xylose. We have examined the influence of three elements that regulate xyl operon expression on diauxic growth and expression of a xylA-lacZ fusion. xylA is 13-fold repressed during growth on glucose. Induction occurs at the onset of the lag phase after glucose is consumed. Inactivation of xylR yields a two-fold increase in expression of xylA on glucose. Deletion of the catabolite responsive element (cre) has a more pronounced effect, reducing glucose repression from 13-fold in the wild type to about 2.5-fold. When xylR and cre are inactivated together a residual two-fold repression of xylA is found. Inactivation of xylR affects diauxic growth by shortening the lag phase from 70 to 40 min. In-frame deletion of ccpA results in the loss of diauxic growth, an increase in doubling time and simultaneous use of both sugars. In contrast, a strain with an inactivated cre site in xylA exhibits diauxic growth without an apparent lag phase on glucose and xylose, whereas fructose and xylose are consumed simultaneously.

References

May 1, 1992·Journal of Bacteriology·T Rygus, W Hillen
Apr 1, 1994·Microbiology·C LindnerM Hecker

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Citations

Aug 31, 2000·Applied and Environmental Microbiology·K A ErlandsonC A Batt
Oct 16, 2007·Journal of Bacteriology·Craig StephensUrs Jenal
Feb 6, 2019·Nature Communications·Jason T BoockJanelle R Thompson
Nov 6, 2015·Applied Biochemistry and Biotechnology·Adilson BosettoRita de Cássia Garcia Simão
May 9, 1998·Infection and Immunity·C L Simpson, R R Russell

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