PMID: 26770430Jan 16, 2016Paper

Control and prevention of myocardial fibrosis using Pyk2-related non-kinase

International Journal of Clinical and Experimental Medicine
Kai YouJia Hao

Abstract

To investigate the antifibrotic effect of Pyk2-related non-kinase (PRNK) and explore the possibility of using adenovirus carrying PRNK gene for targeted inhibition of Pyk2 to treat myocardial fibrosis. Adenovirus carrying PRNK gene was constructed and the angiotention II (Ang II)-induced rat cardiac fibroblasts (CFs) were transfected with the adenovirus. The expressions of PRNK and phosphorylated Pyk2 proteins in CFs were detected. After the preparation of rat model of abdominal aortic stenosis, the rats were infected by the adenovirus expressing PRNK gene. Four groups were set up: sham operation group, PRNK group, drug intervention group and operation group. Myocardial collagen volume fraction (CVF) and perivascular collagen area (PVCA) were measured through Van Gieson (VG) staining, and the content of blue-stained collagen was analyzed by Masson's trichrome staining. TUNEL method was used to detect myocardial cell apoptosis, and secretions of type I and IV collagen in myocardial tissues were detected by ELISA; expressions of PRNK and phosphorylated Pyk2 proteins were detected by Western Blot. Adenoviral vector carrying PRNK gene was successfully constructed; rat CFs were effectively transfected by the adenovirus that expresse...Continue Reading

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