PMID: 6981287Jan 1, 1982Paper

Control of adenosine deaminase levels in human lymphoblasts

Advances in Enzyme Regulation
P E Daddona, W N Kelley

Abstract

High levels of adenosine deaminase (ADA) activity have been associated with normal T cell differentiation and T cell disease, such as acute lymphoblastic leukemia; however, possible mechanisms controlling the level of this enzyme have not been explored. In this study, the properties and rate of turnover of ADA are compared in cultured human T and B lymphoblast cell lines. (1) Relative to B lymphoblasts, the level of ADA activity in extracts of T lymphoblast cell lines (MOLT-4, RPMI-8402, CCRF-CEM and CCRF-HSB-2) is elevated 7- to 14-fold and differs by 2-fold among the T-cell lines. (2) In T and B lymphoblast extracts, the enzyme is apparently identical based on Km for adenosine and deoxyadenosine, Ki for inosine, Vmax for adenosine, S20w, isoelectric pH, and heat stability. Further, by radioimmunoassay the quantity of ADA immunoreactive protein is proportional to the level of enzyme activity in all cell lines studied. (3) Using a purification and selective immunoprecipitation technique, the enzyme turnover could be assessed in cell lines labeled with [35S]methionine. The apparent rate of ADA synthesis, relative to total protein, is 2-fold faster in both T cell lines (RPMI-8402 and CCRF-CEM) than in the B cell lines (MGL-8 and ...Continue Reading

References

May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R L WortmannI H Fox
Sep 1, 1978·The Journal of Clinical Investigation·J F SmythG Yarbro
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B S MitchellW N Kelley
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D A CarsonL Thompson
Sep 1, 1979·Journal of the National Cancer Institute·B E Chechik, J Minowada
Sep 1, 1977·British Journal of Haematology·J L SullivanR J Wedgewood
Sep 1, 1979·The Journal of Clinical Investigation·P E DaddonaW N Kelley
May 1, 1975·British Journal of Cancer·J F Smyth, K R Harrap
Sep 1, 1975·Experimental Cell Research·M S Coleman, J J Hutton
Feb 8, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·T NishizawaT Yoshimura
Apr 1, 1939·The Biochemical Journal·E J Conway, R Cooke

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