Mar 1, 1976

Control of adenylate cyclase from secretory vesicle membranes by beta-adrenergic agents and nerve growth factor

Proceedings of the National Academy of Sciences of the United States of America
O NikodijevicH B Pollard


Adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC] activity of purified secretory vesicle membranes from the adrenal medulla is inhibited by I-isoproterenol and I-epinephrine, as well as by nerve growth factor (NGF). The effect of these agents was found to be dose-dependent and, in the case of the catecholamines, saturable. NGF was active at concentrations as low as 10(-8) M. Oxidized NGF was only minimally active, and insulin was completely inactive. Neither dopamine nor phenylephrine had activity. Inhibition of cyclase by either isoproterenol or epinephrine was blocked by I-propranolol, a specific beta-antagonist, but propranolol by itself had no effect on adenylate cyclase activity. The data indicate that the secretory vesicle membrane has beta-adrenergic receptors coupled to the adenylate cyclase. Propranolol was also found to block the NGF-induced inhibition of cyclase. We conclude that the granule membrane has beta-adrenergic receptors as well as NGF-reactive sites, and that the two may be functionally linked.

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Mentioned in this Paper

Tissue Membrane
Nerve Growth Factor
Antagonist Muscle Action
Catecholamines Measurement
Adrenergic Agonists
Neurite Outgrowth Factors
Epinephrine Measurement
Dopamine Measurement
Beta-adrenergic receptor

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