PMID: 2117756Sep 1, 1990Paper

Control of artifacts in plasma adenosine determinations

Proceedings of the Society for Experimental Biology and Medicine
W P McCann, R E Katholi

Abstract

The literature concerning the role of adenosine (ADO) in physiology reveals no agreement about plasma ADO concentrations and suggests two main sources of error in these determinations: rapid ADO uptake by red blood cells or rapid ADO production from ADO nucleotides, which may be released by any cell lysis or platelet aggregation during plasma preparation. We therefore studied ADO concentrations in plasma from normal human forearm venous blood. ADO was determined by a high-performance liquid chromatographic procedure with a sensitivity of 3 nM (original plasma). Observed ADO concentrations ranged from 894 nM to 8.2 nM depending on the conditions of plasma preparation. In plasma prepared in plastic tubes from 4.5 ml of blood drawn into a plastic syringe containing 1.5 ml of an isotonic stopping solution (pH 7.4) containing heparin (60 units ml), dilazep (40 microM), EGTA (40 mM, EDTA (40 mM), erythro-9-(2-hydroxy-3-nonyl) adenine (40 microM), and alpha, beta-methylene adenosine-5'-diphosphate (525 nM), the plasma ADO concentration was 13.3 +/- 1.88 nM (SE) after correction for a simultaneous ADO recovery determination. The mean ADO recovery was 78% +/- 3.39. The mean plasma ADO concentration found by this method of collection and...Continue Reading

Citations

Jan 1, 1992·Life Sciences·J W PhillisL M Perkins
Nov 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N C BethlenfalvayR E Banks
Feb 9, 1996·Journal of Chromatography. B, Biomedical Applications·C C WangS C Beale
Mar 2, 1999·American Journal of Respiratory and Critical Care Medicine·H SaitoY Kawakami
Apr 18, 2008·European Journal of Pharmacology·Niels P RiksenPaul Smits
Jul 1, 1993·The American Review of Respiratory Disease·A G DriverS J Mustafa

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