Control of brown patch (Rhizoctonia solani) in tall fescue (Festuca arundinacea Schreb.) by host induced gene silencing

Plant Cell Reports
Binbin ZhouRongda Qu

Abstract

Transgenic tall fescue plants expressing RNAi constructs of essential genes of Rhizoctonia solani were resistant to R. solani. Tall fescue (Festuca arundinacea Schreb.) is an important turf and forage grass species widely used for home lawns and on golf courses in North Carolina and other transition zone states in the US. The most serious and frequently occurring disease of tall fescue is brown patch, caused by a basidiomycete fungus, Rhizoctonia solani. This research demonstrates resistance to brown patch disease achieved by the application of host induced gene silencing. We transformed tall fescue with RNAi constructs of four experimentally determined "essential" genes from R. solani (including genes encoding RNA polymerase, importin beta-1 subunit, Cohesin complex subunit Psm1, and a ubiquitin E3 ligase) to suppress expression of those genes inside the fungus and thus inhibit fungal infection. Four gene constructs were tested, and 19 transgenic plants were obtained, among which 12 plants had detectable accumulation of siRNAs of the target genes. In inoculation tests, six plants displayed significantly improved resistance against R. solani. Lesion size was reduced by as much as 90 %. Plants without RNAi accumulation did not s...Continue Reading

References

Jul 11, 1978·Molecular & General Genetics : MGG·M HolstersJ Schell
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Dec 4, 2004·Nucleic Acids Research·Shanfa LuVincent L Chiang
Aug 1, 2006·Journal of Biochemistry·Guillaume van NielGraça Raposo
Jun 9, 2007·Molecular Plant-microbe Interactions : MPMI·Kenny BonfimFrancisco J L Aragão
Nov 9, 2007·Nature Biotechnology·Karl H J Gordon, Peter M Waterhouse
Dec 31, 2010·Molecular Plant-microbe Interactions : MPMI·Chuntao YinScot H Hulbert
Aug 23, 2011·Biochimica Et Biophysica Acta·D Michiel PegtelJaap M Middeldorp
Aug 1, 2012·Molecular Plant Pathology·Emmanuel OgwokTitus Alicai
May 15, 2013·BMC Genomics·Xiaoyi HuangLiang Wang
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Aline KochKarl-Heinz Kogel
Jul 1, 2003·The New Phytologist·Ralf T Voegele, Kurt Mendgen

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Citations

Oct 18, 2016·Molecular Plant Pathology·Yin Song, Bart P H J Thomma
Oct 3, 2017·Pest Management Science·Ana Karla MachadoKim E Hammond-Kosack
Feb 7, 2020·Molecular Plant Pathology·Mengying Wang, Ralph A Dean
May 19, 2019·Functional & Integrative Genomics·Srayan GhoshGopaljee Jha
Oct 9, 2020·Molecular Plant-microbe Interactions : MPMI·Srayan GhoshGopaljee Jha

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