PMID: 9530097Apr 8, 1998Paper

Control of Ca2+ wave propagation in mouse pancreatic acinar cells

The American Journal of Physiology
F PfeifferI Schulz

Abstract

We have investigated control mechanisms involved in the propagation of agonist-induced Ca2+ waves in isolated mouse pancreatic acinar cells. Using a confocal laser-scanning microscope, we were able to show that maximal stimulation of cells with acetylcholine (ACh, 500 nM) or bombesin (1 nM) caused an initial Ca2+ release of comparable amounts with both agonists at the luminal cell pole. Subsequent Ca2+ spreading to the basolateral membrane was faster with ACh (17.3 +/- 5.4 microns/s) than with bombesin (8.0 +/- 2.2 microns/s). The speed of bombesin-induced Ca2+ waves could be increased up to the speed of ACh-induced Ca2+ waves by inhibition of protein kinase C (PKC). Activation of PKC significantly decreased the speed of ACh-induced Ca2+ waves but had only little effect on bombesin-evoked Ca2+ waves. Within 3 s after stimulation, production of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] was higher in the presence of ACh compared with bombesin, whereas bombesin induced higher levels of diacylglycerol (DAG) than ACh. These data suggest that the slower propagation speed of bombesin-induced Ca2+ waves is due to higher activation of PKC in the presence of bombesin compared with ACh. The higher increase in bombesin-compared with ACh-...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·J A Gordon
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Sep 1, 1987·The American Journal of Physiology·E RousseauG Meissner
Jan 1, 1995·Advances in Second Messenger and Phosphoprotein Research·D E Clapham, J Sneyd
Apr 1, 1995·The American Journal of Physiology·C Bentz, J P Hildebrandt
Mar 15, 1996·The Journal of Biological Chemistry·D L BennettM D Bootman
Oct 4, 1996·The Journal of Biological Chemistry·X XuS Muallem

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Citations

Sep 21, 2000·The Journal of Biological Chemistry·A GonzálezA Schmid
May 15, 2002·Journal of Physiology and Biochemistry·A González, G M Salido
Oct 7, 2006·Current Opinion in Gastroenterology·J A Williams
Sep 24, 1999·Biological Chemistry·I SchulzA Schmid
Jun 9, 2000·Annual Review of Physiology·R Greger
Dec 24, 2002·Microsurgery·Alan E FreelandTodd G Nick
Oct 21, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Anna-Karin H Hultgård-EkwallHelge Rask-Andersen
Sep 11, 2003·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Anna Koralewska-MakárBerndt Ehinger
May 5, 2004·The Journal of Biological Chemistry·Qin LiShmuel Muallem
Oct 29, 2004·Nature Reviews. Cancer·Carl-Henrik HeldinArne Ostman
Jun 17, 2003·The Journal of Biological Chemistry·Lutz SternfeldIrene Schulz
Mar 27, 2002·Connective Tissue Research·H MartelJ L Bert
Nov 26, 2002·Acta Physiologica Scandinavica·V V Iversen, R K Reed
Dec 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kaushik ParthasarathiJahar Bhattacharya
Mar 5, 2011·The Journal of Biological Chemistry·Abrahim I OrabiSohail Z Husain
Aug 23, 2017·Scientific Reports·Vahid ReyhaniKristofer Rubin
Sep 9, 1998·The American Journal of Physiology·A GonzálezI Schulz
Oct 16, 2002·Cell Calcium·L Jaffe
Oct 5, 2010·Cell Calcium·Lionel F Jaffe

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