Control of cell fate determination by p21ras/Ras1, an essential component of torso signaling in Drosophila

Genes & Development
X LuN Perrimon

Abstract

Determination of cell fate at the posterior termini of the Drosophila embryo is specified by the activation of the torso (tor) receptor tyrosine kinase. This signaling pathway is mediated by the serine/threonine kinase D-raf and a protein tyrosine phosphatase corkscrew (csw). We found that expression of an activated form of Ras1 during oogenesis resulted in embryos with tor gain-of-function phenotypes. To demonstrate that p21ras/Ras1 mediates tor signaling, we injected mammalian p21ras variants into early Drosophila embryos. We found that the injection of activated p21v-ras rescued the maternal-effect phenotypes of both tor and csw null mutations. These rescuing effects of p21v-ras are dependent on the presence of maternally derived D-raf activity. In addition, wild-type embryos show a terminal-class phenotype resembling csw when injected with p21rasN17, a dominant-negative form of p21ras. Furthermore, we have analyzed the maternal-effect phenotype of Son of sevenless (Sos), a positive regulator of Ras1, and showed that embryos derived from germ cells lacking Sos+ activity exhibit a terminal-class phenotype. Our study demonstrates that the Drosophila p21ras, encoded by Ras1, is an intrinsic component of the tor signaling pathwa...Continue Reading

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Citations

Oct 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S T EzerZ Lev
Dec 1, 1995·Molecular Reproduction and Development·N PerrimonL A Perkins
May 1, 1997·Developmental Biology·T Lee, D J Montell
Mar 1, 1994·Cancer Metastasis Reviews·N G Williams, T M Roberts
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Oct 1, 1994·Mechanisms of Development·J CasanovaG Struhl
Nov 1, 1994·Trends in Biochemical Sciences·N Perrimon, C Desplan
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·A M BennettB G Neel
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·H UmanoffR Kucherlapati
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·W SwatF W Alt
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·H R MottS L Campbell
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·U GaykoN Perrimon
Feb 1, 1994·Current Opinion in Genetics & Development·B Dickson, E Hafen
Aug 1, 1993·Current Opinion in Genetics & Development·T B Kornberg, T Tabata
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May 16, 2007·PLoS Genetics·Carole SeumSéverine Bontron
Feb 27, 2007·Current Biology : CB·Joseph K ParkQinghua Liu
Apr 6, 2004·Developmental Biology·Fernando Bejarano, Ana Busturia
May 18, 2017·Nature Chemical Biology·Lukasz Kowalik, James K Chen
Apr 1, 1994·Current Opinion in Cell Biology·N Perrimon
Jan 5, 1996·The Journal of Biological Chemistry·J K DruganS L Campbell

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