PMID: 9419353Feb 21, 1998Paper

Control of direction of flagellar rotation in bacterial chemotaxis

Proceedings of the National Academy of Sciences of the United States of America
B E ScharfH C Berg

Abstract

The motile behavior of the bacterium Escherichia coli depends on the direction of rotation of its flagellar motors. Binding of the phosphorylated signaling molecule CheY to a motor component FliM is known to enhance clockwise rotation. It is difficult to study this interaction in vivo, because the dynamics of phosphorylation of CheY by its kinase CheA and the hydrolysis of CheY (accelerated by CheZ) are not under direct experimental control. Here, we examine instead the interaction with the flagellar motor of a double mutant CheY13DK106YW that is active without phosphorylation. The behavioral assays were carried out on tethered cells lacking CheA and CheZ. The effects of variation in intracellular concentration of the mutant protein were highly nonlinear. However, they can be explained by a thermal isomerization model in which the free energies of clockwise and counterclockwise states depend linearly on the amount of CheY bound.

References

Nov 1, 1977·Biophysical Journal·H C Berg, E M Purcell
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R B BourretM I Simon
Sep 1, 1989·Journal of Bacteriology·C M HamiltonS R Kushner
Nov 1, 1989·Journal of Bacteriology·S C Kuo, D E Koshland
Jul 1, 1988·Journal of Bacteriology·G Kuwajima
Dec 1, 1986·Journal of Bacteriology·G KuwajimaE Kondo
Jan 1, 1973·Journal of Bacteriology·M Silverman, M Simon
Jan 1, 1967·Journal of Bacteriology·J B ArmstrongM M Dahl
Apr 1, 1983·Journal of Bacteriology·S M BlockH C Berg
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·J C WangK Becherer
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R A Smith, J S Parkinson
Nov 1, 1982·Cell·S M BlockH C Berg
Nov 1, 1993·Biophysical Journal·H C Berg, L Turner
Jul 1, 1993·Journal of Bacteriology·S Slater, R Maurer
May 1, 1993·Molecular Biology of the Cell·D BrayM I Simon
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M WelchM Eisenbach
Jun 1, 1996·Molecular Microbiology·M Eisenbach
Oct 1, 1996·Biophysical Journal·L TurnerH C Berg
Nov 1, 1996·Journal of Bacteriology·A G GarzaM D Manson
Jun 15, 2000·Journal of Bacteriology·K C BoeschR B Bourret

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Citations

Aug 7, 2012·Bulletin of Mathematical Biology·Xiangrong Xin, Hans G Othmer
Feb 29, 2000·Biophysical Journal·R M Berry, J P Armitage
May 22, 2003·Journal of Molecular Biology·Thomas S ShimizuDennis Bray
Feb 5, 2000·Current Biology : CB·J Stock, M Levit
Jan 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Junhua Yuan, Howard C Berg
Aug 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yuhai Tu
May 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Nicolas J DelalezJudith P Armitage
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Junhua YuanHoward C Berg
Jun 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Taejin L MinYann R Chemla
Nov 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Pushkar P LeleHoward C Berg
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·R JasujaS Khan
Mar 23, 2011·Physical Biology·Thierry MoraNed S Wingreen
Aug 4, 1998·The EMBO Journal·U AlonJ B Stock
Oct 17, 2002·Protein Science : a Publication of the Protein Society·Sandra Da ReJeffry B Stock
Jun 4, 2013·Applied and Environmental Microbiology·Chen QianKeng-Hwee Chiam
Jun 15, 2000·Journal of Bacteriology·K C BoeschR B Bourret
Jul 11, 2002·Journal of Bacteriology·Daniël T VerhammeKlaas J Hellingwerf
Aug 22, 2013·MBio·Murray J TippingJudith P Armitage
Dec 26, 2002·Annual Review of Biochemistry·Howard C Berg
Sep 29, 2011·BMC Systems Biology·Diana Clausznitzer, Robert G Endres
May 10, 2006·PLoS Computational Biology·Karen Lipkow
Jun 2, 2012·PLoS Computational Biology·Qi MaFan Bai
Apr 16, 2011·PloS One·Tuba AltindalXiao-Lun Wu
Jul 31, 2012·PloS One·Henry G ZotNguyen Van Minh
Sep 4, 2012·International Journal of Molecular Sciences·Masayoshi Nishiyama, Seiji Kojima
Apr 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Eli Paster, William S Ryu
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dennis Bray
Apr 4, 2014·Journal of Molecular Modeling·Ahmet YildirimTsjerk A Wassenaar
Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·Victor Sourjik, Howard C Berg
Apr 3, 2014·Science Signaling·Hajime FukuokaAkihiko Ishijima
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·R RamakrishnanR B Bourret
Mar 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Susan M Butler, Andrew Camilli
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·A D SamuelH C Berg
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·M SchusterR B Bourret
Oct 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·Fangbin WangHoward C Berg
Jan 1, 2008·Journal of Biophysics·I DigelG M Artmann
Feb 9, 2010·Current Opinion in Microbiology·Birgit E Scharf
Oct 28, 2009·Mathematical Biosciences·Ricardo C H del RosarioDieter Oesterhelt
Mar 31, 2015·Cell·Lynne ChantranupongDavid M Sabatini
May 27, 2009·Journal of Molecular Biology·Junhua YuanHoward C Berg

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