Control of domain swapping in bovine odorant-binding protein

The Biochemical Journal
Roberto RamoniChristian Cambillau

Abstract

As revealed by the X-ray structure, bovine odorant-binding protein (OBPb) is a domain swapped dimer [Tegoni, Ramoni, Bignetti, Spinelli and Cambillau (1996) Nat. Struct. Biol. 3, 863-867; Bianchet, Bains, Petosi, Pevsner, Snyder, Monaco and Amzel (1996) Nat. Struct. Biol. 3, 934-939]. This contrasts with all known mammalian OBPs, which are monomers, and in particular with porcine OBP (OBPp), sharing 42.3% identity with OBPb. By the mechanism of domain swapping, monomers are proposed to evolve into dimers and oligomers, as observed in human prion. Comparison of bovine and porcine OBP sequences pointed at OBPp glycine 121, in the hinge linking the beta-barrel to the alpha-helix. The absence of this residue in OBPb might explain why the normal lipocalin beta-turn is not formed. In order to decipher the domain swapping determinants we have produced a mutant of OBPb in which a glycine residue was inserted after position 121, and a mutant of OBPp in which glycine 121 was deleted. The latter mutation did not result in dimerization, while OBPb-121Gly+ became monomeric, suggesting that domain swapping was reversed. Careful structural analysis revealed that besides the presence of a glycine in the hinge, the dimer interface formed by the...Continue Reading

Citations

Oct 30, 2012·Analytical and Bioanalytical Chemistry·Federica BianchiRoberto Ramoni
Sep 18, 2004·European Journal of Biochemistry·Florence VincentChristian Cambillau
Jul 7, 2011·International Journal of Molecular Sciences·Eugenia PolveriniRoberto Favilla
Feb 14, 2008·Biochimica Et Biophysica Acta·Roberto RamoniMariella Tegoni
Jun 1, 2006·Proteins·Arun S KonagurthuArthur M Lesk
Mar 1, 2006·Journal of Plant Physiology·Joanna GrzybKazimierz Strzałka
Mar 8, 2003·Structure·Frederic RousseauLaura S Itzhaki
Apr 13, 2007·Journal of Structural Biology·Alberto MazziniRoberto Favilla
Sep 10, 2010·Veterinary Pathology·G B MitchellJ L Caswell
Apr 12, 2016·Scientific Reports·Hanbin JeongChangwook Lee
Apr 20, 2004·FEBS Letters·Silvia SpinelliChristian Cambillau
Sep 6, 2018·PloS One·Alessandro CapoMaria Staiano
Jul 20, 2006·The Journal of Biological Chemistry·Eric HajjarCharles H Robert
Sep 18, 2003·The Journal of Biological Chemistry·Emmanuelle LaurineJean-Michel Verdier
Feb 6, 2021·Critical Reviews in Biotechnology·Filipa GonçalvesArtur Cavaco-Paulo
Dec 17, 2019·Biophysical Chemistry·Homero Gómez-VelascoEnrique García-Hernández
Dec 14, 2002·Biochimica Et Biophysica Acta·Alberto MazziniRoberto Favilla
Sep 5, 2021·Biological Reviews of the Cambridge Philosophical Society·Paolo Pelosi, Wolfgang Knoll

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