Control of epithelial ion transport by Cl- and PDZ proteins

The Journal of Membrane Biology
R SchreiberK Kunzelmann

Abstract

Inhibition of epithelial Na+ channels (ENaC) by the cystic fibrosis transmembrane conductance regulator (CFTR) has been demonstrated previously. Recent studies suggested a role of cytosolic Cl- for the interaction of CFTR with ENaC, when studied in Xenopus oocytes. In the present study we demonstrate that the Na+ / H+ -exchanger regulator factor (NHERF) controls expression of CFTR in mouse collecting duct cells. Inhibition of NHERF largely attenuates CFTR expression, which is paralleled by enhanced Ca(2+) -dependent Cl- secretion and augmented Na+ absorption by the ENaC. It is further demonstrated that epithelial Na+ absorption and ENaC are inhibited by cytosolic Cl- and that stimulation by secretagogues enhances the intracellular Cl- concentration. Thus, the data provide a clue to the question, how epithelial cells can operate as both absorptive and secretory units: Increase in intracellular Cl- during activation of secretion will inhibit ENaC and switch epithelial transport from salt absorption to Cl- secretion.

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Citations

Nov 2, 2004·Pflügers Archiv : European journal of physiology·Gabriele AdamKarl Kunzelmann
Jul 2, 2009·Clinical and Experimental Pharmacology & Physiology·Lauren M O'MullaneAnuwat Dinudom
Mar 9, 2013·Cellular and Molecular Life Sciences : CMLS·Haijie YuAndrea Fleig
Jan 22, 2014·The Journal of Physiology·Eun Suk JungMin Goo Lee
Nov 14, 2007·Chest·Scott H Donaldson, Richard C Boucher
Dec 27, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Yukio KatoAkira Tsuji
Mar 19, 2008·Journal of Cellular Physiology·Yuchun Gu
Nov 12, 2010·Molecular Membrane Biology·Diana FariaMargarida D Amaral
Sep 24, 2004·Physiology·Karl Kunzelmann, Brendan McMorran
Oct 27, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K KunzelmannR Schreiber
Jul 20, 2005·The Journal of Biological Chemistry·Tanja BachhuberKarl Kunzelmann

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