PMID: 11607300Jun 15, 1992Paper

Control of excision frequency of maize transposable element Ds in Petunia protoplasts

Proceedings of the National Academy of Sciences of the United States of America
D BeckerP Starlinger

Abstract

The complete coding region of maize transposable element Ac and truncated but active derivatives of it were placed under the control of promoters of different strength and tested for the ability to excise transposable element Ds from a beta-glucuronidase reporter gene in a cotransfection assay in Petunia protoplasts. The highest excision values (5% of the protoplasts able to express the beta-glucuronidase gene in a control experiment) were observed with a truncated version of the Ac coding region under the control of the 2' promoter. The weak Ac promoter is sufficient to give rise to excision values not much lower than those found with much stronger promoters such as the 2' and nos promoters. A decrease in excision frequency was observed when translation of the Ac coding region was hindered by out-of-frame ATG codons in addition to the use of weak promoters. Increasing the level of Ac transposase thus does not seem to be sufficient to raise the level of Ds excision observed in this system and possibly also in maize. Therefore another factor limits the excision of Ds elements. Previously, it was reported that in tobacco cells the deletion of Ds sequence between base pairs 186 and 245 led to a decrease of the Ds excision frequenc...Continue Reading

References

Feb 1, 1992·Molecular & General Genetics : MGG·C M RommensJ Hille
Feb 1, 1991·Molecular & General Genetics : MGG·H FusswinkelR Kunze
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J YenI M Verma
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M G Li, P Starlinger
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G CouplandP Starlinger
Jan 1, 1989·Annual Review of Genetics·A GierlP A Peterson
Jul 24, 1987·Nucleic Acids Research·R TöpferH H Steinbiss
Jan 1, 1951·Cold Spring Harbor Symposia on Quantitative Biology·B McCLINTOCK
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C KonczA A Szalay
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D Schwartz

❮ Previous
Next ❯

Citations

Nov 27, 1995·Molecular & General Genetics : MGG·S Chatterjee, P Starlinger
Sep 25, 1996·Molecular & General Genetics : MGG·P GrappinM A Grandbastien
Jan 31, 2009·BMC Genomics·Katina Lazarow, Stephanie Lütticke
Nov 12, 2009·Journal of Integrative Plant Biology·Shaohong QuGuo-Liang Wang
Sep 5, 2006·Genetics·Alexander EmelyanovSerguei Parinov
May 25, 1994·Molecular & General Genetics : MGG·G N RudenkoJ Hille

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
G CouplandP Starlinger
Proceedings of the National Academy of Sciences of the United States of America
M G Li, P Starlinger
Proceedings of the National Academy of Sciences of the United States of America
R KunzeR Lütticke
© 2021 Meta ULC. All rights reserved