Control of flagellar gene regulation in Legionella pneumophila and its relation to growth phase.

Journal of Bacteriology
Christiane Albert-WeissenbergerCarmen Buchrieser

Abstract

The bacterial pathogen Legionella pneumophila responds to environmental changes by differentiation. At least two forms are well described: replicative bacteria are avirulent; in contrast, transmissive bacteria express virulence traits and flagella. Phenotypic analysis, Western blotting, and electron microscopy of mutants of the regulatory genes encoding RpoN, FleQ, FleR, and FliA demonstrated that flagellin expression is strongly repressed and that the mutants are nonflagellated in the transmissive phase. Transcriptome analyses elucidated that RpoN, together with FleQ, enhances transcription of 14 out of 31 flagellar class II genes, which code for the basal body, hook, and regulatory proteins. Unexpectedly, FleQ independent of RpoN enhances the transcription of fliA encoding sigma 28. Expression analysis of a fliA mutant showed that FliA activates three out of the five remaining flagellar class III genes and the flagellar class IV genes. Surprisingly, FleR does not induce but inhibits expression of at least 14 flagellar class III genes on the transcriptional level. Thus, we propose that flagellar class II genes are controlled by FleQ and RpoN, whereas the transcription of the class III gene fliA is controlled in a FleQ-dependen...Continue Reading

References

Dec 1, 1984·The Journal of Cell Biology·M A Horwitz, F R Maxfield
Sep 1, 1980·The Journal of Clinical Investigation·M A Horwitz, S C Silverstein
Oct 1, 1994·Journal of Bacteriology·D Foster-HartnettR G Kranz
Jun 20, 1998·Journal of Molecular Biology·P J CullenR G Kranz
Jun 25, 1998·Infection and Immunity·B Byrne, M S Swanson
Aug 14, 1999·Molecular Microbiology·B K Hammer, M S Swanson
Oct 6, 2000·Annual Review of Microbiology·M S Swanson, B K Hammer
Nov 9, 2000·The Journal of Experimental Medicine·S Sturgill-Koszycki, M S Swanson
Mar 12, 2002·Traffic·Michele S SwansonEsteban Fernandez-Moreia
Apr 23, 2002·Molecular Microbiology·Brian K HammerMichele S Swanson
Jul 19, 2003·International Journal of Medical Microbiology : IJMM·Klaus Heuner, Michael Steinert
Nov 18, 2003·Molecular Microbiology·Ari B Molofsky, Michele S Swanson
Jul 1, 2004·Molecular Microbiology·Ari B Molofsky, Michele S Swanson
Sep 28, 2004·Science·Minchen ChienJames J Russo
Jul 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·John-Demian SauerMichele S Swanson
Feb 21, 2006·Current Opinion in Microbiology·Linda L McCarter
Apr 12, 2006·The Journal of Experimental Medicine·Ari B MolofskyMichele S Swanson
Dec 29, 2006·Cellular and Molecular Life Sciences : CMLS·C Albert-WeissenbergerC Buchrieser
Nov 13, 2007·Journal of Bacteriology·Alan J Wolfe, Karen L Visick
Mar 12, 2008·Molecular Microbiology·Sivaramesh WigneshwerarajMartin Buck
May 20, 2008·Molecular Microbiology·Jason W Hickman, Caroline S Harwood
Dec 2, 2008·Molecular Microbiology·Zachary D DalebrouxMichele S Swanson

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Citations

Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tamara J O'ConnorRalph R Isberg
Apr 12, 2012·Applied and Environmental Microbiology·Matthew D MastropaoloStuart B Levy
Mar 31, 2010·Infection and Immunity·Rachel L EdwardsMichele S Swanson
Apr 24, 2012·Journal of Bacteriology·Erin B PurcellRita Tamayo
Apr 24, 2012·Pathologie-biologie·H TrabelsiA Ayadi
Dec 24, 2011·Water Research·Charles BodetYann Héchard
Mar 5, 2010·Molecular Microbiology·Zachary D DalebrouxMichele S Swanson
Aug 27, 2015·BMC Genomics·Laam LiSébastien P Faucher
Aug 13, 2013·International Journal of Medical Microbiology : IJMM·Elzbieta BrzuszkiewiczKlaus Heuner
Jan 8, 2015·Frontiers in Microbiology·Peter RobertsonRafael A Garduño
Aug 6, 2016·Biofouling·Sophie PécastaingsAnne Vianney
Jul 28, 2016·FEBS Letters·Wolfgang Eisenreich, Klaus Heuner
Feb 9, 2017·Diseases of Aquatic Organisms·Gabriela P CarrilSergio H Marshall
Feb 7, 2018·Frontiers in Cellular and Infection Microbiology·Giulia OlivaCarmen Buchrieser
Jun 7, 2014·The Journal of Biological Chemistry·Eva SchunderKlaus Heuner
Jul 19, 2012·Médecine sciences : M/S·Yvan JamillouxFlorence Ader
Nov 7, 2017·Frontiers in Cellular and Infection Microbiology·Sandra Appelt, Klaus Heuner
Dec 1, 2017·Current Opinion in Microbiology·Nicolas PersonnicHubert Hilbi
Dec 4, 2021·Environmental Microbiology·Antonio Leal-MoralesFernando Govantes

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