Dec 7, 2016

Control of Gene Expression by RNA Binding Protein Action on Alternative Translation Initiation Sites

PLoS Computational Biology
Angela ReAlessandro Quattrone

Abstract

Transcript levels do not faithfully predict protein levels, due to post-transcriptional regulation of gene expression mediated by RNA binding proteins (RBPs) and non-coding RNAs. We developed a multivariate linear regression model integrating RBP levels and predicted RBP-mRNA regulatory interactions from matched transcript and protein datasets. RBPs significantly improved the accuracy in predicting protein abundance of a portion of the total modeled mRNAs in three panels of tissues and cells and for different methods employed in the detection of mRNA and protein. The presence of upstream translation initiation sites (uTISs) at the mRNA 5' untranslated regions was strongly associated with improvement in predictive accuracy. On the basis of these observations, we propose that the recently discovered widespread uTISs in the human genome can be a previously unappreciated substrate of translational control mediated by RBPs.

Mentioned in this Paper

Exertion
Untranslated Regions
HEK293 Cells
Post-Transcriptional Regulation
Genome
RNA, Untranslated
Sequence Determinations, RNA
MT-TA gene
Transcription, Genetic
Fluorescence-Activated Cell Sorting

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