Control of germline stem cell division frequency--a novel, developmentally regulated role for epidermal growth factor signaling.

PloS One
Benjamin B ParrottCordula Schulz

Abstract

Exploring adult stem cell dynamics in normal and disease states is crucial to both better understanding their in vivo role and better realizing their therapeutic potential. Here we address the division frequency of Germline Stem Cells (GSCs) in testes of Drosophila melanogaster. We show that GSC division frequency is under genetic control of the highly conserved Epidermal Growth Factor (EGF) signaling pathway. When EGF signaling was attenuated, we detected a two-fold increase in the percentage of GSCs in mitotic division compared to GSCs in control animals. Ex vivo and in vivo experiments using a marker for cells in S-phase of the cell cycle showed that the GSCs in EGF mutant testes divide faster than GSCs in control testes. The increased mitotic activity of GSCs in EGF mutants was rescued by restoring EGF signaling in the GSCs, and reproduced in testes from animals with soma-depleted EGF-Receptor (EGFR). Interestingly, EGF attenuation specifically increased the GSC division frequency in adult testes, but not in larval testes. Furthermore, GSCs in testes with tumors resulting from the perturbation of other conserved signaling pathways divided at normal frequencies. We conclude that EGF signaling from the GSCs to the CySCs norma...Continue Reading

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Citations

Feb 12, 2014·Seminars in Cell & Developmental Biology·Fenghua Zeng, Raymond C Harris
Feb 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Natalia M TulinaAmita Sehgal
Oct 23, 2012·Spermatogenesis·Richard Zoller, Cordula Schulz
Oct 23, 2012·Spermatogenesis·Erika L MatunisMargaret de Cuevas
Oct 23, 2012·Spermatogenesis·Cindy LimXin Chen
Aug 8, 2014·Frontiers in Genetics·Marion MorelColette Dissous
Oct 17, 2017·The EMBO Journal·Patricia Rojas-RíosMartine Simonelig
Jan 1, 2020·International Journal of Molecular Sciences·Sarah Mele, Travis K Johnson
Jan 31, 2018·Genetics·Ryan J GleasonXin Chen
Feb 22, 2021·Developmental Biology·Velinda Vidaurre, Xin Chen

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Methods Mentioned

BETA
transgenic
Fluorescence
dissection

Software Mentioned

Axiovision Rel
Axiovision

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