Control of hypertrophic scar growth using antibody-targeted photolysis

The Journal of Surgical Research
S F WolfortM L Yarmush

Abstract

Hypertrophic scar is marked by excess collagen accumulation secondary to an increased vascularization response in the scar and an increase in fibroblast cell density. It is currently the most debilitating long-term complication of the surviving burn patient, and at present, there is no routinely effective form of therapy. In this study, we investigated the potential use of antibody-targeted photolysis (ATPL) in treating hypertrophic scars. An immunoconjugate consisting of a photosensitizer (Sn-chlorin e6) linked to a monoclonal antibody that binds to human myofibroblasts (PR2D3) was prepared, which in response to photoactivation produces singlet oxygen in close proximity to the target cell surface. The model used for these studies consisted of 1-mm 3 human hypertrophic scar tissue implants in athymic mice. These implants increase approximately 20-fold in volume over a period of 15 days. Four days after implantation immunoconjugate was injected directly into scar implants allowed to diffuse throughout for 24 hr before implants were illuminated with laser light at 630 nm (120 J/cm 2). ATPL treatment caused a significant reduction in total growth compared to the untreated controls (P < 0.05). No effect was observed when an irrelev...Continue Reading

Citations

Aug 13, 2008·Journal of Burn Care & Research : Official Publication of the American Burn Association·Kathy Q ZhuLoren H Engrav
Jan 9, 2013·Biotechnology and Bioengineering·Alexander GolbergMartin L Yarmush
Jul 16, 1999·Lasers in Surgery and Medicine·S G ParekhR R Anderson
Nov 25, 2000·Journal of Biomechanical Engineering·A J Fowler, M P Menguc
Jan 1, 1997·Lasers in Surgery and Medicine·S R ReikenM L Yarmush
Oct 30, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Kathy Q ZhuLoren H Engrav
Apr 18, 2007·Journal of Burn Care & Research : Official Publication of the American Burn Association·Youfu XieLoren H Engrav
Aug 1, 2007·Journal of Burn Care & Research : Official Publication of the American Burn Association·Loren H EngravEdward E Tredget
Jul 6, 2005·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·T MaischC Abels
Mar 17, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Aaron J BullousRoss W Boyle
Apr 27, 2000·Progress in Biophysics and Molecular Biology·A S SobolevA A Rosenkranz

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