Control of intestinal promoter activity of the cellular migratory regulator gene ELMO3 by CDX2 and SP1

Journal of Cellular Biochemistry
Mehmet CoskunJ T Troelsen

Abstract

An important aspect of the cellular differentiation in the intestine is the migration of epithelial cells from the crypt to the villus tip. As homeodomaine transcription factor CDX2 has been suggested to influence cell migration, we performed a genome-wide promoter analysis for CDX2 binding in the differentiated human intestinal cancer cell line Caco-2 in order to identify CDX2-regulated genes involved in cellular migration. The engulfment and cell motility 3 (ELMO3) gene was identified as a potential CDX2 target gene. ELMO3 is an essential upstream regulator of the GTP-binding protein RAC during cell migration. However, no information is available about the transcriptional regulation of the ELMO3 gene. The aim of this study was to investigate the potential role of CDX2 in the regulation of the ELMO3 promoter activity. Electrophoretic mobility shift assays showed that CDX2 bound to conserved CDX2 sequences and mutations of the CDX2-binding sites, significantly reduced the promoter activity. Reporter gene assays demonstrated that the region mediating ELMO3 basal transcriptional activity to be located between -270 and -31 bp. Sequence analysis revealed no typical TATA-box, but four GC-rich sequences. In vitro analyses (electropho...Continue Reading

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Citations

Feb 14, 2012·Biochimica Et Biophysica Acta·Mehmet CoskunJesper Thorvald Troelsen
Dec 4, 2010·Biochimica Et Biophysica Acta·Mehmet CoskunOle Haagen Nielsen
Oct 19, 2016·PloS One·Steffen JørgensenJesper T Troelsen
Dec 22, 2016·International Journal of Molecular Sciences·Hui-Yun PengDian Gao
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Feb 9, 2013·Carcinogenesis·Anders Krüger OlsenJesper Thorvald Troelsen
Mar 3, 2018·International Journal of Molecular Sciences·Signe Regner MichaelsenLasse Sommer Kristensen
Oct 31, 2018·Pathology Oncology Research : POR·Ulana KotowskiGregor Heiduschka
Oct 23, 2018·BioMed Research International·Yan HuDian Gao

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