Control of ion distortion in field asymmetric waveform ion mobility spectrometry via variation of dispersion field and gas temperature.

Analytical Chemistry
Errol W RobinsonRichard D Smith

Abstract

Field asymmetric waveform ion mobility spectrometry (FAIMS) has emerged as an analytical tool of broad utility, especially in conjunction with mass spectrometry. Of particular promise is the use of FAIMS and 2-D ion mobility methods that combine FAIMS with conventional IMS to resolve and characterize protein and other macromolecular conformers. However, FAIMS operation requires a strong electric field, and ions are inevitably heated by energetic collisions with buffer gas molecules. This may induce ion isomerization or dissociation, which distort the separation properties of FAIMS (and subsequent stages) or reduce instrumental sensitivity. As FAIMS employs a periodic waveform, whether those processes are controlled by ion temperature at maximum or average field intensity has been debated. Here we address this issue by measuring the unfolding of compact ubiquitin ion geometries as a function of waveform amplitude (dispersion field, E(D)) and gas temperature, T. The field heating is quantified by matching the dependences of structural transitions on E(D) and T: increasing E(D) from 12 to 16 or from 16 to 20 kV/cm is equivalent to heating the (N2) gas by approximately 15-25 degrees C. The magnitude of field heating for any E(D) ca...Continue Reading

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Citations

Apr 25, 2012·Journal of the American Society for Mass Spectrometry·Georgios PapadopoulosThomas R Rizzo
Jul 30, 2010·Analytical Chemistry·Alexandre A ShvartsburgRichard D Smith
Dec 2, 2010·Analytical Chemistry·Alexandre A Shvartsburg, Richard D Smith
Aug 15, 2012·Analytical Chemistry·Alexandre A Shvartsburg, Richard D Smith
Dec 19, 2012·Analytical Chemistry·Alexandre A Shvartsburg, Richard D Smith
Nov 7, 2008·Analytical Chemistry·Alexandre A Shvartsburg, Richard D Smith
Jul 9, 2009·Analytical Chemistry·Alexandre A ShvartsburgBilly Boyle
Aug 28, 2009·Analytical Chemistry·Alexandre A ShvartsburgDanielle Toutoungi
Feb 16, 2010·Analytical Chemistry·Alexandre A ShvartsburgRichard D Smith
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Jan 13, 2010·European Journal of Mass Spectrometry·Bradley B SchneiderErkinjon G Nazarov
Feb 5, 2016·Journal of the American Society for Mass Spectrometry·Helen J Cooper
Nov 1, 2016·Journal of the American Society for Mass Spectrometry·Andrew J Creese, Helen J Cooper
Dec 25, 2013·Analytical Chemistry·Emily R SchenkFrancisco Fernandez-Lima
Feb 2, 2019·Mass Spectrometry Reviews·Valérie GabelicaThomas Wyttenbach
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Mar 19, 2021·Journal of the American Society for Mass Spectrometry·Christian IeritanoW Scott Hopkins
May 4, 2017·Journal of the American Society for Mass Spectrometry·Andrew P BowmanAlexandre A Shvartsburg
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Jul 17, 2013·Analytical Chemistry·Alexandre A Shvartsburg, Richard D Smith
Jun 30, 2019·Analytical Chemistry·Alexandre A ShvartsburgRoger Giles
May 11, 2018·Accounts of Chemical Research·Michael Z Kamrath, Thomas R Rizzo
Sep 4, 2020·Journal of the American Society for Mass Spectrometry·Pratima PathakAlexandre A Shvartsburg

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