Control of Listeria monocytogenes infection requires classical IL-6 signaling in myeloid cells

PloS One
Karsten LückeHans-Willi Mittrücker

Abstract

IL-6 is required for the response of mice against Listeria monocytogenes. Control of infection depends on classical IL-6 signaling via membrane IL-6Rα, but IL-6 target cells and protective mechanisms remain unclear. We used mice with IL-6Rα-deficiency in T cells (Il6rafl/fl×CD4cre) or myeloid cells (Il6rafl/fl×LysMcre) to define the role of these cells in IL-6-mediated protection. Abrogation of IL-6Rα in T cells did not interfere with bacteria control and induction of TH1 and CD8+ T-cell responses. IL-6Rα-deficiency in myeloid cells caused significant defects in listeria control. This defect was not associated with reduced recruitment of granulocytes and inflammatory monocytes, and both cell populations were activated and not impaired in cytokine production. However, IL-6Rα-deficient inflammatory monocytes displayed diminished expression of IL-4Rα and of CD38, a protein required for phagocytosis and innate control of listeria. In vitro studies revealed that IL-4 and IL-6 cooperated in induction of CD38. In listeria-infected mice, phagocytic activity of inflammatory monocytes correlated with CD38 expression levels on cells and inflammatory monocytes of Il6rafl/fl×LysMcre mice were significantly impaired in phagocytosis. In concl...Continue Reading

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Citations

Jan 23, 2020·Cells·Estibaliz Glaría, Annabel F Valledor
Oct 25, 2020·American Journal of Reproductive Immunology : AJRI·Carleigh E FedorkaBarry A Ball
May 28, 2019·Microbiology Spectrum·Sarah E F D'Orazio
Jun 20, 2021·International Journal of Medical Microbiology : IJMM·Yurong ZhuBing Hu

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BETA
PCR
flow cytometry
PMA
transgenic

Software Mentioned

GraphPad
ImageJ
Prism

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