Control of maximum sarcoplasmic reticulum Ca load in intact ferret ventricular myocytes. Effects Of thapsigargin and isoproterenol

The Journal of General Physiology
K S GinsburgD M Bers

Abstract

In steady state, the Ca content of the sarcoplasmic reticulum (SR) of cardiac myocytes is determined by a balance among influx and efflux pathways. The SR Ca content may be limited mainly by the ATP-supplied chemical potential that is inherent in the gradient between SR and cytosol. That is, forward Ca pumping from cytosol to SR may be opposed by energetically conservative reverse pumping dependent on intra-SR free [Ca]. On the other hand, SR Ca loading may be limited by dissipative pathways (pump slippage and/or pump-independent leak). To assess how SR Ca content is limited, we loaded voltage-clamped ferret ventricular myocytes cumulatively with known amounts of Ca via L-type Ca channels (ICa), using Na-free solutions to prevent Na/Ca exchange. We then measured the maximal resulting caffeine-released SR Ca content under control conditions, as well as when SR Ca pumping was accelerated by isoproterenol (1 micro M) or slowed by thapsigargin (0.2-0.4 micro M). Under control conditions, SR Ca content reached a limit of 137 micro mol.liter cytosol-1 (nonmitochondrial volume) when measured by integrating caffeine-induced Na/Ca exchange currents lintegraINaCaXdt) and of 119 micro mol.liter cytosol-1 when measured using fluorescence s...Continue Reading

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Citations

Jun 24, 2003·Journal of Molecular and Cellular Cardiology·Zhi SuWilliam H Barry
Feb 7, 2001·The Journal of General Physiology·C R WeberD M Bers
Apr 25, 2012·Clinical and Experimental Pharmacology & Physiology·Angela F DulhuntyNicole A Beard
Nov 20, 2013·Annual Review of Physiology·Donald M Bers
Jun 22, 2000·Journal of Molecular and Cellular Cardiology·C I SpencerJ R Berlin
Jul 25, 2007·Clinical and Experimental Pharmacology & Physiology·Derek R Laver
Sep 11, 2012·Journal of Molecular and Cellular Cardiology·Ariel L EscobarJosefina Ramos-Franco
Nov 23, 2000·The Journal of Biological Chemistry·T R ShannonD M Bers
Jun 18, 2004·Annals of the New York Academy of Sciences·Thomas R Shannon, Donald M Bers
Jul 26, 2018·International Journal of Molecular Sciences·Tatiana M VinogradovaEdward G Lakatta
Aug 19, 2000·Circulation Research·D M Bers
Oct 5, 2002·Circulation Research·Thomas R ShannonDonald M Bers
Apr 29, 2005·The Japanese Journal of Physiology·Takeo TanaamiWilliam H Barry
May 2, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Beatriz M R CarvalhoJosé W M Bassani
Nov 5, 2014·Physiology·Holly A Shiels, Gina L J Galli
Jul 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·H KohzukiM Takaki
Jan 27, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nitisha HiranandaniPaul M L Janssen
Aug 28, 2020·The Journal of Experimental Biology·Tatiana S FilatovaHolly A Shiels

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