PMID: 7372705May 1, 1980Paper

Control of membrane fusion in exocytosis. Physiological studies on a Paramecium mutant blocked in the final step of the trichocyst extrusion process

The Journal of Cell Biology
J BeissonM Rossignol

Abstract

Previous studies on exocytosis in Paramecium using mutants affecting trichocyst extrusion permitted us to analyze the assembly and function of three intramembrane particle arrays ("ring" and "rosette" in the plasma membrane, "annulus" in the trichocyst membrane) involved in the interaction between these two membranes. Using a conditional mutation, nd9, which blocks rosette assembly and prevents exocytosis at the nonpermissive temperature, we have analyzed the effect of temperature on the secretory capacity of nd9 cells. By combining several techniques (physiological studies, microinjections, inhibition of fatty acid synthesis, and freeze-fracture analysis) we demonstrate (a) that the product of the mutated allele nd9 is not thermolabile but that its activity is dependent upon temperature-induced changes in the membrane lipid composition and (b) that the product of the nd9 locus is a diffusible cytoplasmic component whose interaction with both plasma membrane and trichocyst membrane is required for rosette assembly and exocytosis. The data provide physiological evidence for the existence of a molecular complex(es) linking the two membranes and involved in the control of membrane fusion; we discuss the possible nature and functio...Continue Reading

References

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Citations

Jun 24, 2000·Biochimie·L VayssiéJ Cohen
Mar 1, 1987·The Journal of Cell Biology·F RuizJ Beisson
Dec 1, 1981·The Journal of Cell Biology·J J Rauh, D L Nelson
Feb 1, 1982·The Journal of Cell Biology·N HagaC Kung
Jul 1, 1983·The Journal of Cell Biology·D M Gilligan, B H Satir
Nov 29, 1991·European Journal of Protistology·G KnollH Plattner
Sep 10, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Bernard Tandler
Jul 1, 1989·The Journal of protozoology·B H Satir
Aug 14, 2003·Biochimica Et Biophysica Acta·Helmut Plattner, Roland Kissmehl
Oct 20, 2001·Journal of Experimental Psychology. Human Perception and Performance·P G AmazeenP J Beek
Apr 22, 2009·Molecular and Cellular Biology·Eva-Maria LadenburgerHelmut Plattner
May 29, 2000·European Journal of Biochemistry·A Galvani, L Sperling
Apr 1, 1996·Histochemistry and Cell Biology·M KnochelR D Burgoyne
Jan 27, 2021·Nature Microbiology·Eleonora AquiliniMaryse Lebrun
May 29, 2021·Trends in Parasitology·Daniela Sparvoli, Maryse Lebrun
Aug 19, 2021·Nature Communications·Shrawan Kumar MageswaranYi-Wei Chang

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