PMID: 2125490Nov 6, 1990Paper

Control of mucin synthesis: the peptide portion of synthetic O-glycopeptide substrates influences the activity of O-glycan core 1 UDPgalactose:N-acetyl-alpha-galactosaminyl-R beta 3-galactosyltransferase

Biochemistry
I BrockhausenHans Paulsen

Abstract

Synthetic O-glycopeptides containing one or two GalNAc residues attached to Ser or Thr were used as substrates to investigate the effect of peptide structure on the activity of crude preparations of UDP-Gal:GalNAc alpha-R beta 3-Gal-transferase from pig stomach and pig and rat colonic mucosa and of a partially purified enzyme preparation from rat liver. High-performance liquid chromatography used to separate enzyme products revealed that uncharged glycopeptides with an acetyl group at the amino-terminal end and a tertiary butyl or an amide group at the carboxy-terminal end were resistant to proteolysis in crude preparations. The activity of beta 3-Gal-transferase varied with the sequence and length of the peptide portion of the substrate, the presence of protecting groups, the attachment site of GalNAc, and the number of GalNAc residues in the substrate. The presence and position of Pro had little effect on enzyme activity; ionizing groups near the GalNAc unit interfered with enzyme activity. Since the GalNAc-Thr moieties in many of these O-glycopeptides have been shown to assume similar rigid conformations, the variation in enzyme activity indicates that the beta 3-Gal-transferase recognizes both the peptide and carbohydrate m...Continue Reading

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Citations

Sep 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P L Devine, I F McKenzie
Aug 17, 1994·Molecular and Cellular Biochemistry·A M WuA Herp
Dec 1, 1994·Critical Reviews in Oncology/hematology·T LesuffleurF X Real
Dec 2, 1999·Biochimica Et Biophysica Acta·I Brockhausen
Jun 1, 1994·Environmental Health Perspectives·J M Samet, P W Cheng
Jan 25, 2011·Angewandte Chemie·Tongzhong JuRichard D Cummings
Sep 1, 1992·Current Problems in Cancer·J M Jessup, G E Gallick
Jan 1, 1993·Critical Reviews in Clinical Laboratory Sciences·I Brockhausen
Mar 27, 2018·Glycobiology·Ieva Bagdonaite, Hans H Wandall
Oct 24, 2001·The Journal of Biological Chemistry·Tongzhong JuWilliam M Canfield
Jun 1, 1994·International Journal of Peptide and Protein Research·M MeldalK Bock

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