Control of normal differentiation of myeloid leukemic cells. VIII. Induction of differentiation to mature granulocytes in mass culture

Journal of Cellular Physiology
E Fibach, L Sachs

Abstract

There are three types of myeloid leukemic cells, IR+D, IR+D- and IR-D-. IR+D+ cells were induced to differentiate to granulocytes in mass culture in liquid medium by conditioned medium (CM) from cultures of lungs from mice injected with endotoxin. About 90% of the leukemic cells were induced to differentiate, 50% to mature granulocytes and 40% to intermediate stages. An efficient induction of granulocyte differentiation was also obtained with CM from primary cultures of rat embryo or human spleen and there was a lower activity with CM from various other sources. IR+D- cells were induced to differentiate to about 20% cells with intermediate stages but not to mature granulocytes; IR-D- cells could not be induced to differentiate to intermediate or mature stages. IR+D+ cells were induced to form intermediate stages of granulocyte differentiation, to phagocytose and to attach to the surface of the Petri dish, three days after incubation with CM. Optimum induction of mature granulocytes required six more days incubation with CM. Mature granulocytes induced from leukemic cells showed cytochemical properties and a morphology in the electron microscope similar to that of normal mature granulocytes. These induced granulocytes did not fo...Continue Reading

References

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Citations

Jan 1, 1983·Advances in Enzyme Regulation·E L SchwartzA C Sartorelli
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J I Azumi, L Sachs
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J Lotem, L Sachs
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S J CollinsR C Gallo
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Lotem, L Sachs
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·L Sachs
Dec 1, 1978·British Journal of Haematology·L Sachs
Oct 3, 1978·Virchows Archiv. A, Pathological Anatomy and Histology·T KimotoY Nishioka
Sep 1, 1988·Journal of Clinical Pharmacology·E L Schwartz, P H Wiernik
Mar 19, 2014·Journal of Theoretical Biology·Sang-Woo LeeYoshihiro Morishita
Jul 18, 2002·Annals of the New York Academy of Sciences·Theodore T PuckRobert Johnson
Sep 15, 1981·International Journal of Cancer. Journal International Du Cancer·J Lotem, L Sachs
Dec 1, 1986·Cell Biophysics·L Sachs
Aug 21, 1987·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·L Sachs
Jan 15, 1984·International Journal of Cancer. Journal International Du Cancer·J Lotem, L Sachs
Dec 15, 1983·International Journal of Cancer. Journal International Du Cancer·J Lotem, L Sachs
Jul 15, 1983·International Journal of Cancer. Journal International Du Cancer·J Lotem, L Sachs
Nov 15, 1979·International Journal of Cancer. Journal International Du Cancer·D Metcalf
Jun 15, 1980·International Journal of Cancer. Journal International Du Cancer·J LotemL Sachs
Nov 15, 1980·International Journal of Cancer. Journal International Du Cancer·A Falk, L Sachs
Feb 15, 1980·International Journal of Cancer. Journal International Du Cancer·D Metcalf

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