PMID: 9652414Jul 4, 1998Paper

Control of oxidative phosphorylation, gluconeogenesis, ureagenesis and ATP turnover in isolated perfused rat liver analyzed by top-down metabolic control analysis

European Journal of Biochemistry
S SobollG C Brown

Abstract

We have analyzed the control exerted by the pathways of oxidative phosphorylation, gluconeogenesis, ureagenesis, and maintenance ATP consumption over each other's rates in isolated, perfused rat liver using top-down metabolic control analysis. The livers from fasted rats were perfused with 3-hydroxybutyrate as respiratory substrate, lactate as substrate for gluconeogenesis, and ammonium as substrate for urea synthesis, in conditions where these pathways were only linked by their common intermediates: ATP, ADP, and Pi. The rates of oxygen consumption, glucose and urea synthesis were measured continuously. The pathways were perturbed either by adding specific inhibitors or by adding new pathways that consumed ATP, and the relative changes in pathway rates were used to calculate the flux control coefficients of each pathway over all pathway rates. When the liver was in a relatively inactive metabolic state, where ATP was only being used by the maintenance ATP-consuming pathways, then essentially all the control over ATP production and consumption was located in the maintenance ATP consumers with ATP production having no control. Whereas, when the liver was in a highly active state using extra ATP for both glucose and urea synthesi...Continue Reading

Citations

May 30, 2001·Advanced Drug Delivery Reviews·I E Scheffler
Sep 1, 1999·European Journal of Biochemistry·E K Ainscow, M D Brand
Dec 3, 1999·European Journal of Biochemistry·E K Ainscow, M D Brand
Oct 6, 2011·The Journal of Biological Chemistry·Lijun LiuMarcus P Cooper
Aug 30, 2007·BMC Physiology·Jean-Louis GallisMarie-Christine Beauvieux
Apr 30, 2010·BMC Systems Biology·Klaus MaierKlaus Mauch
Dec 27, 2011·Critical Reviews in Biomedical Engineering·Mehmet A OrmanMarianthi G Ierapetritou
May 23, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Young Suk JoJohan Auwerx
Aug 30, 2005·Metabolism: Clinical and Experimental·Michael E Bizeau, Michael J Pagliassotti
Apr 8, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hanne HoitzingNick S Jones
Oct 7, 2003·Annual Review of Biomedical Engineering·Martin L Yarmush, Scott Banta
Apr 28, 2005·The Journal of Experimental Biology·Raul K Suarez, Charles A Darveau

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