Abstract
The level of p53 is markedly increased in human cells in response to expression of the Ad12 E1A proteins and, quite separately, to the Ad12 E1B 54K protein. The behaviour of p53 in these two circumstances has been examined using A549 cells infected with Ad12 dl620 (a mutant virus which does not express the larger E1B protein and is replication-defective) and human skin fibroblasts expressing the Ad12 E1B 54K protein (HSF 54K). In normal and E1A-expressing A549 cells, p53 is located predominantly in the nucleus, whereas in the HSF 54K cells it is primarily cytoplasmic as is the Ad12 E1B 54K protein. The half-life of p53 is increased in Ad12 dl620-infected A549 cells from about 10 min (in uninfected cells) to 2 hr. The half-life of p53 in HSF 54K cells is even longer-probably in excess of 48 hr. The capacity of p53 to regulate transcription was assessed using a transfected CAT construct linked to p53-responsive elements. p53 transcriptional activity was very low in the HSF 54K cells and in human embryo kidney cells expressing the Ad12 E1B 54K protein (and p53) at high level. It was, however, dramatically increased in response to the p53 expressed as a result of E1A expression. Additionally, MDM2 was present at low level in the HS...Continue Reading
Citations
Jan 18, 2012·Human Gene Therapy·Taeyoung KooChae-Ok Yun
Sep 6, 2000·The EMBO Journal·A S TurnellP H Gallimore
Jun 18, 2011·Journal of Virology·Qiang LiDaiqing Liao
Nov 3, 2006·Journal of Virology·Jennifer L Woo, Arnold J Berk
Feb 13, 2009·Journal of Virology·Andrew N Blackford, Roger J A Grand
May 18, 2001·Journal of Virology·B R DixA W Braithwaite
Jan 3, 2001·Journal of Virology·E QueridoM R Morisson
Nov 20, 2002·Journal of Virology·Sara J EdwardsAntony W Braithwaite
May 28, 1998·Virology·R J GrandA S Turnell
Feb 14, 2006·Cancer Gene Therapy·H SauthoffJ G Hay
Jul 14, 1998·Somatic Cell and Molecular Genetics·H L MacArthurS Bacchetti
Feb 3, 2000·Oncogene·F G HuttonR J Grand
Feb 19, 1999·Oncogene·R J GrandP H Gallimore
Feb 2, 1999·Oncogene·R J GrandP H Gallimore
Aug 5, 2000·The Journal of Biological Chemistry·M OtsukaM Omata
Jan 9, 1999·Journal of Virology·D BoivinP E Branton
Mar 14, 1998·Journal of Virology·E A Fortunato, D H Spector
Aug 8, 1998·Journal of Virology·R C MarcellusP E Branton
Feb 11, 1999·Journal of Virology·A S TurnellP H Gallimore
Feb 15, 2001·Seminars in Cancer Biology·F McCormick
Sep 1, 1997·Journal of Virology·P H GallimoreJ Parkhill
May 1, 1997·Journal of Virology·E QueridoP E Branton
May 1, 1997·Journal of Virology·E QueridoP E Branton
Oct 20, 2018·ACS Chemical Biology·Nils SchneiderMatthias Meier