Control of peroxisome proliferator-activated receptor gamma2 stability and activity by SUMOylation

Obesity Research
Z Elizabeth Floyd, Jacqueline M Stephens

Abstract

To determine whether small ubiquitin-related modifier (SUMO)ylation of lysine 107 plays a role in regulating the activity of peroxisome proliferator-activated receptor gamma (PPARgamma). Transient expression of wild-type and K107R-PPARgamma2 in the NIH 3T3 fibroblast cell line was carried out in conjunction with half-life studies, luciferase activity assays, and indirect immunofluorescence localization studies. Additional in vitro analysis was carried out using recombinant SUMOylation pathway proteins along with in vitro transcribed and translated wild-type or K107R-PPARgamma2 to examine the SUMO-1 modification state of wild-type and SUMO-deficient K107R-PPARgamma2. While examining PPARgamma2 for potential ubiquitylation sites, we identified a strong consensus site for SUMO modification that contains lysine 107. In vitro, SUMOylation studies showed that lysine 107 of PPARgamma2 is a major SUMOylation site and that at least one other SUMOylation site is present in PPARgamma. In addition, our results demonstrated that SUMO-1 affects PPARgamma stability and transcriptional activity but not the nuclear localization of PPARgamma. These results indicated that SUMOylation plays a role in regulating PPARgamma, both indirectly and direc...Continue Reading

References

May 15, 1994·Genes & Development·P TontonozB M Spiegelman
Jul 25, 1997·The Journal of Biological Chemistry·L FajasJ Auwerx
Apr 5, 2000·The Journal of Biological Chemistry·S HauserB M Spiegelman
Oct 14, 2000·Annual Review of Cell and Developmental Biology·E D Rosen, B M Spiegelman
Oct 14, 2000·Annual Review of Cell and Developmental Biology·F Melchior
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·H PoukkaJ J Palvimo
Jan 2, 2001·The Journal of Biological Chemistry·M S RodriguezR T Hay
Mar 27, 2001·Nature Reviews. Molecular Cell Biology·S MüllerS Jentsch
Jul 19, 2001·The Journal of Biological Chemistry·E D Rosen, B M Spiegelman
Aug 7, 2001·Frontiers in Bioscience : a Journal and Virtual Library·A P DennisZ Nawaz
Dec 26, 2001·The Journal of Biological Chemistry·Z Elizabeth Floyd, Jacqueline M Stephens
May 25, 2002·Molecular and Cellular Biology·Bonnie J DerooTrevor K Archer
Jul 30, 2002·The Biochemical Journal·Sha TianOlli A Jänne
Aug 24, 2002·Endocrinology·Yves Le DreanDenis Michel
Oct 2, 2002·The Journal of Biological Chemistry·Masahide TojoMitsuyoshi Nakao
Jan 17, 2003·The Journal of Biological Chemistry·Anne ChauchereauEdwin Milgrom
Oct 3, 2003·Nature Cell Biology·J Russell Lipford, Raymond J Deshaies

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Citations

Feb 4, 2009·Antioxidants & Redox Signaling·Zheng XuShannon Wing Ngor Au
Nov 1, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Kristine M Wadosky, Monte S Willis
Jul 12, 2005·Expert Opinion on Investigational Drugs·Martin H Fenner, Elena Elstner
Sep 8, 2009·Molecular and Cellular Endocrinology·Ursula A White, Jacqueline M Stephens
Sep 16, 2006·Molecular Cell·Xiang-Jiao Yang, Serge Grégoire
Sep 24, 2009·Molecular and Cellular Endocrinology·Constantinos Christodoulides, Antonio Vidal-Puig
Jul 16, 2010·Gene·Dongyao YanHee-Jeong Im
Mar 9, 2005·Molecular & Cellular Proteomics : MCP·James P DeLanyJeffrey M Gimble
Feb 2, 2013·Circulation Research·Frederick W Quelle, Curt D Sigmund
Oct 26, 2006·Journal of Cellular Physiology·Julie S MoyersAlexei Kharitonenkov
May 24, 2008·Obesity·Fang HeJacqueline M Stephens
Apr 27, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brahmchetna BediRuxana T Sadikot
Jul 16, 2020·Physiological Reviews·Hui-Ming Chang, Edward T H Yeh
Oct 1, 2010·Endocrinology·Angelo CignarelliFrancesco Giorgino
Dec 29, 2012·Endocrinology·Laura MikkonenOlli A Jänne
Jun 27, 2013·American Journal of Physiology. Endocrinology and Metabolism·Chongben ZhangZhen Yan
Nov 16, 2006·The Journal of Biological Chemistry·Kenichi YokotaHiroshi Itoh
Dec 11, 2008·The Biochemical Journal·Moritz HentschkeUwe Borgmeyer
Nov 14, 2018·Proceedings of the National Academy of Sciences of the United States of America·Takeshi KatafuchiSteven A Kliewer
Jun 14, 2018·International Journal of Molecular Sciences·Reinhard Brunmeir, Feng Xu
Oct 20, 2020·Frontiers of Medicine·Deyu ZhangQinong Ye
Jun 14, 2005·Biochimica Et Biophysica Acta·Jan NedergaardBarbara Cannon

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