Control of phosphatase and tensin homolog (PTEN) gene expression in normal and neoplastic thyroid cells

Endocrinology
Gianluca TellGiuseppe Damante

Abstract

The lipid phosphatase, phosphatase and tensin homolog (PTEN), is a key element in controlling cell growth and survival and has a well established role as tumor suppressor protein in many neoplasia. Several data indicate that silencing of PTEN gene expression may be relevant in follicular thyroid cell transformation. Thus, in the present study regulation of PTEN gene expression in thyroid cells was investigated. Cotransfection experiments indicated that in normal FRTL-5 rat thyroid cells, PTEN promoter activity was increased by overexpression of the transcription factor early growth response protein-1 (Egr-1). Moreover, Western blot experiments indicated that when Egr-1 expression was up-regulated by treating FRTL-5 cells with H2O2, an increase in PTEN expression was also observed. TSH induced opposite modifications on PTEN and Egr-1 protein levels. Moreover, acute or chronic TSH stimulation determined distinct effects. In fact, acute TSH stimulation (30 and 60 min) induced a decrease in PTEN, but an increase in Egr-1 protein levels. These effects were cAMP dependent; in fact, they were mimicked by forskolin. A chronic TSH treatment (5 d) stimulated PTEN protein expression, whereas Egr-1 protein was down-regulated. In contrast t...Continue Reading

References

Jan 1, 1979·International Review of Cytology. Supplement·F S Ambesi-Impiombato, H G Coon
Jun 1, 1990·Journal of Molecular Endocrinology·M ChambardO Chabaud
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·F S Ambesi-ImpiombatoH G Coon
Jan 1, 1995·Progress in Nucleic Acid Research and Molecular Biology·A Gashler, V P Sukhatme
May 1, 1997·The Journal of Clinical Endocrinology and Metabolism·F ArturiS Filetti
Oct 28, 1998·Genes, Chromosomes & Cancer·N HalachmiD Sidransky
Nov 14, 1998·Biochemical and Biophysical Research Communications·G TellG Damante
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L C Cantley, B G Neel
Sep 9, 2000·Journal of Medical Genetics·J T CelebiM Peacocke
Apr 21, 2001·Journal of Endocrinological Investigation·D RussoG Tell
Jun 8, 2001·Annual Review of Biochemistry·T MaehamaJ E Dixon
Sep 8, 2001·Molecular Cell·V StambolicT W Mak
Dec 1, 2001·The Journal of Biological Chemistry·Lindsey D MayoDavid B Donner
Mar 5, 2002·American Journal of Human Genetics·Kristin A Waite, Charis Eng
Aug 31, 2002·Genes, Chromosomes & Cancer·Tony FriskCatharina Larsson
Aug 26, 2003·Human Mutation·Charis Eng
Jan 20, 2004·Molecular and Cellular Biology·Krishna Murthi VasudevanVivek M Rangnekar

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Citations

May 5, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Laura CesarattoGianluca Tell
May 14, 2010·Apoptosis : an International Journal on Programmed Cell Death·Rongrong HouQiuhe Ji
Aug 3, 2006·Nature Reviews. Drug Discovery·Sandrine FaivreEric Raymond
Nov 17, 2005·Oncogene·Zenglin Lian, Antonio Di Cristofano
Jan 21, 2006·International Journal of Cancer. Journal International Du Cancer·Rosemary E TeresiCharis Eng
Mar 8, 2008·Free Radical Research·Damiano FantiniGianluca Tell
May 21, 2014·The Journal of Clinical Endocrinology and Metabolism·Bin Li, Terry J Smith
Jun 19, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gerard Hernandez-MirMandy J McGeachy
Apr 15, 2020·Clinical Endocrinology·Feng WeiBingyin Shi

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