PMID: 9539713May 16, 1998Paper

Control of phosphatidylserine biosynthesis through phosphatidylserine-mediated inhibition of phosphatidylserine synthase I in Chinese hamster ovary cells

Proceedings of the National Academy of Sciences of the United States of America
O KugeM Nishijima

Abstract

Phosphatidylserine (PtdSer) synthesis in Chinese hamster ovary (CHO) cells occurs through the exchange of L-serine with the base moiety of phosphatidylcholine or phosphatidylethanolamine. The synthesis is depressed on the addition of PtdSer to the culture medium. A CHO cell mutant named mutant 29, whose PtdSer biosynthesis is highly resistant to this depression by exogenous PtdSer, has been isolated from CHO-K1 cells. In the present study, the PtdSer-resistant PtdSer biosynthesis in the mutant was traced to a point mutation in the PtdSer synthase I gene, pssA, resulting in the replacement of Arg-95 of the synthase by lysine. Introduction of the mutant pssA cDNA, but not the wild-type pssA cDNA, into CHO-K1 cells induced the PtdSer-resistant PtdSer biosynthesis. In a cell-free system, the serine base-exchange activity of the wild-type pssA-transfected cells was inhibited by PtdSer, but that of the mutant pssA-transfected cells was resistant to the inhibition. Like the mutant 29 cells, the mutant pssA-transfected cells grown without exogenous PtdSer exhibited an approximately 2-fold increase in the cellular PtdSer level compared with that in CHO-K1 cells, although the wild-type pssA-transfected cells did not exhibit such a signif...Continue Reading

References

Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·O KugeY Akamatsu
Oct 1, 1974·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W D Marggraf, F A Anderer
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D R Voelker
Aug 14, 1999·The Journal of Biological Chemistry·O KugeM Nishijima
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

❮ Previous
Next ❯

Citations

Jun 28, 2005·Progress in Lipid Research·Jean E Vance, Rineke Steenbergen
Aug 12, 2009·The Journal of Biological Chemistry·Roberta LeonardiSuzanne Jackowski
Nov 12, 2010·The Journal of Biological Chemistry·Guergana TassevaJean E Vance
Nov 22, 2011·Journal of Lipid Research·Shin-ya MoritaTomohiro Terada
Aug 2, 2006·Biological & Pharmaceutical Bulletin·Akiko Yamaji-Hasegawa, Masafumi Tsujimoto
Jul 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mohammed AkbarHee-Yong Kim
Feb 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Edouard MullarkyLewis C Cantley
Mar 16, 2011·The Journal of Cell Biology·Bill X HuangHee-Yong Kim
Jul 30, 2008·Endothelium : Journal of Endothelial Cell Research·Julie K FreedAndrew S Greene
May 29, 2013·IUBMB Life·Andrés M Cardozo Gizzi, Beatriz L Caputto
Mar 9, 2011·Progress in Lipid Research·Martin HermanssonPentti Somerharju
Apr 5, 2016·Proceedings of the National Academy of Sciences of the United States of America·Mira SohnTamas Balla
May 3, 2015·Nutrition·Michael J Glade, Kyl Smith
Nov 20, 2012·Biochimica Et Biophysica Acta·Ville KainuPentti Somerharju
Sep 11, 2012·Biochimica Et Biophysica Acta·Jean E Vance, Guergana Tasseva
Jul 6, 2014·Progress in Lipid Research·Hee-Yong KimArthur A Spector
Jul 16, 2008·Biochimica Et Biophysica Acta·Inna FreikmanEitan Fibach
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jean E Vance, Dennis E Vance
Mar 23, 2017·Nature·Zuzana KeckesovaRobert A Weinberg
Jan 26, 2017·Journal of Lipid Research·Muthukumar KannanWilliam A Prinz
Jan 18, 2018·American Journal of Medical Genetics. Part a·Juliette PiardLionel Van Maldergem
May 22, 2001·Environmental Health Perspectives·R T RileyA H Merrill
Aug 13, 2003·The Journal of Biological Chemistry·Osamu KugeMasahiro Nishijima
Aug 15, 2020·Frontiers in Cell and Developmental Biology·Nicolas-Frédéric LippGuillaume Drin
Jul 14, 2000·The Journal of Biological Chemistry·A L HenneberryC R McMaster
Oct 9, 2019·Annual Review of Cell and Developmental Biology·Tamas BallaJoshua Pemberton
Aug 14, 1999·The Journal of Biological Chemistry·O KugeM Nishijima
Oct 4, 2002·The Journal of Biological Chemistry·Martin O BergoStephen G Young
Nov 19, 2008·The Biochemical Journal·Shiho TomohiroOsamu Kuge
Feb 26, 2015·The Journal of Biological Chemistry·Krishna Chaithanya BatchuPentti Somerharju
Jul 28, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Taki Nishimura, Christopher J Stefan
Nov 19, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Atsuko K Kimura, Tomohiro Kimura
Mar 19, 2021·Cellular Oncology (Dordrecht)·Mingxue LiHua Li
Sep 14, 2021·Protein Science : a Publication of the Protein Society·Non Miyata, Osamu Kuge

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.