PMID: 392504Nov 1, 1979

Control of phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli by energization of the cell membrane

Proceedings of the National Academy of Sciences of the United States of America
E ReiderM Schweiger

Abstract

The phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli is inhibited by the energized of the membrane. This was shown in intact cells as well as in membrane vesicles. Relaxation of the proton gradient by uncouplers stimulated the uptake of sugars via the phosphotransferase system in aerobically cultured cells. No such effect was seen in anaerobic cells, apparently because the cell membrane of these cells is poorly energized. Energization by respiration of D-lactate or ascorbate inhibited the phosphotransferase uptake system in membrane vesicles. This inhibition was reversed by the addition of cyanide. Oxamate, a specific inhibitor of lactate dehydrogenase, prevented the inhibitory effect of D-lactate. Membrane vesicles prepared from a cytochrome-less mutant were not energized by D-lactate oxidation and the phosphotransferase uptake system was not inhibited.

References

Apr 21, 1975·Biochemical and Biophysical Research Communications·F F CampoJ M Ramírez
Jan 1, 1978·Annual Review of Biochemistry·H C Andersen
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A Peterkofsky, C Gazdar
Dec 6, 1974·Science·H R Kaback
Dec 1, 1958·Biochimica Et Biophysica Acta·R F HILL

Citations

Feb 1, 1984·Journal of Bioenergetics and Biomembranes·B Labedan, L Letellier
Oct 15, 1983·Archives of Biochemistry and Biophysics·P C Loewen, H E Richter
Jul 1, 1996·Research in Microbiology·M H Saier, M Crasnier
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W N Konings, G T Robillard
Jan 15, 1996·European Journal of Biochemistry·J M RohwerH V Westerhoff
May 9, 2012·Journal of Bioscience and Bioengineering·Nobutaka Nakashima, Tomohiro Tamura
Jun 1, 1981·European Journal of Biochemistry·M Michels, E P Bakker
Dec 1, 1979·European Journal of Biochemistry·E F WagnerM Schweiger
Jul 13, 2000·Critical Reviews in Biotechnology·J C Diaz Ricci, M E Hernández
Apr 1, 1995·European Journal of Biochemistry·M BonhiversL Letellier

Related Concepts

Plasma Membrane
Isocyanides
Cytochromes
Respiratory Chain
Alkalescens-Dispar Group
Lactates
Methylglucosides
Methylglycosides
Phosphoenolpyruvate
Phosphotransferases

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