PMID: 558342Apr 1, 1977Paper

Control of protein synthesis in Semliki forest virus-infected cells

Journal of Virology
B E Lachmi, L Kääriäinen

Abstract

Protein synthesis in Semliki forest virus-infected chicken embryo cells was studied by labeling them with [35S]methionine for short periods at different times after infection, with or without synchronization of protein synthesis by the hypertonic block technique. The rate of host-cell protein synthesis declined almost linearly in inverse correlation to the increase in the amount of virus specific RNA. At 5.5 h postinfection, the host-cell protein synthesis was reduced by about 70%. The viral structural proteins were detectable with certainty at 3.5 h postinfection, and their rate of synthesis increased linearly parallel to the amount of their messenger, the 26S RNA. This suggests that the rate of synthesis of the structural proteins is controlled at the level of transcription. The rate of synthesis of the virus-specific nonstructural proteins attained its maximum between 3 and 4 h postinfection and declined thereafter, wheras the amount of their messenger, the 42S RNA, continued to increase linearly in the cells. Thus, the messenger activity of the 42S RNA is reduced in the late phase of infection compared with its activity in the early phase.

References

Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D L NussG Koch
Oct 5, 1975·Journal of Molecular Biology·J C Clegg, S I Kennedy
Feb 1, 1976·The Journal of General Virology
Jul 12, 1976·Biochemical and Biophysical Research Communications·N Glanville, I Ulmanen
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·B E Lachmi, L Kääriäinen
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·N GlanvilleA E Smith
Apr 21, 1975·Biochemical and Biophysical Research Communications·G Temple, H F Lodish
Aug 1, 1975·Journal of Virology·S Keränen, L Kääriäinen
May 15, 1974·Journal of Molecular Biology·J L SaborioG Koch
Jun 25, 1974·Journal of Molecular Biology·D T Simmons, J H Strauss
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R Cancedda, M J Schlesinger
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Sep 1, 1974·Journal of Virology·D T Simmons, J H Strauss
Apr 1, 1971·Journal of Virology·J G Levin, R M Friedman
Oct 1, 1966·Virology·B W Burge, E R Pfefferkorn

❮ Previous
Next ❯

Citations

Jan 1, 1992·Archives of Virology·C R Dâmaso, N Moussatché
Dec 1, 1980·European Journal of Biochemistry·P LehtovaaraL Philipson
Mar 27, 2001·Journal of Virology·P KujalaL Kääriäinen
Feb 9, 2000·Journal of Virology·S I IsmailM Uden
May 1, 1990·Journal of Virology·J PeränenL Kääriäinen
Jan 1, 1980·Pharmacology & Therapeutics·L Carrasco, A E Smith
May 23, 1978·Biochimica Et Biophysica Acta·N GlanvilleL Kääriäinen
Jul 25, 1979·Journal of Molecular Biology·T K FreyJ H Strauss

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.