Control of protozoa contamination and lipid accumulation in Neochloris oleoabundans culture: Effects of pH and dissolved inorganic carbon

Bioresource Technology
Licheng PengMatt Laporte

Abstract

Combined effects of pH (i.e., 7.5, 8.5, and 9.5) and bicarbonate (i.e., 0, 80 and 160mM NaHCO3) on lipid accumulation and on biological contaminant viability in a protozoa-contaminated culture of the freshwater microalga Neochloris oleoabundans were studied. Cultures grown in the media containing 160mM NaHCO3 at pH 9.5 obtained the highest biomass concentration (DCWmax=1.32g/L), lipid content (LC=327mg/g), which corresponded to a lipid productivity of 56mg/(L·d), and the culture was protozoa free one day after inoculation. Other cultures, 160mM NaHCO3 at pH 8.5 (DCWmax=1.32g/L, LC=223mg/g), and 80mM NaHCO3 at pH 9.5 (DCWmax=1.25g/L, LC=264mg/g) could delay protozoan growth, but not inhibit it completely. These results suggest 160mM NaHCO3 or slightly above at pH levels of 8.5-9.5 may be used in outdoor cultivation processes of freshwater N. oleoabundans to control protozoa contamination while maintain a high lipid content.

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Citations

Jun 9, 2017·Biotechnology Progress·Changhao LiDankui Liao
Nov 14, 2019·Applied Microbiology and Biotechnology·Denisse MolinaCarlos Ricardo Soccol
Dec 1, 2018·Environmental Science and Pollution Research International·Zeqing BaoChristopher Q Lan
Jan 4, 2019·World Journal of Microbiology & Biotechnology·Gloria Sánchez-GalvánItzel A Juárez
Jul 19, 2019·Environmental Science and Pollution Research International·Neha MaheshwariShaili Srivastava

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