PMID: 3768955Oct 24, 1986Paper

Control of rDNA replication in Tetrahymena involves a cis-acting upstream repeat of a promoter element

Cell
D LarsonE Orias

Abstract

A novel genetic scheme was used to isolate mutants altered in the formation or maintenance of amplified rDNA in the Tetrahymena macronucleus. One such mutant had a cis-acting rDNA mutation that affected the ability of mutant rDNA molecules to replicate in macronuclei in the presence of a wild-type (B strain) rDNA. The mutant rDNA was lost from these heterozygous macronuclei during vegetative cell divisions, although it was maintained normally in the homozygous or hemizygous state. In contrast, wild-type macronuclear rDNA of the C3 strain used to obtain the mutant outreplicated B strain rDNA in B/C3 heterozygote macronuclei. Sequence differences were found between wild-type B and C3 and mutant C3 rDNAs in the replication origin region, changing an upstream repeat of a highly conserved rRNA promoter element. We propose that the various rDNA alleles differentially compete for limiting amounts of trans-acting factors that bind to these enhancer-like repeats and positively regulate rDNA replication.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 5, 1979·Journal of Molecular Biology·N Din, J Engberg
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P J BrunsE H Blackburn
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M Lusky, M R Botchan
Apr 1, 1986·Molecular and Cellular Biology·J J LiT Kelly
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M M Tondravi, M C Yao
Oct 1, 1973·Experimental Cell Research·C T Roberts, E Orias
Nov 15, 1984·Nature·J de VilliersR Kamen
May 1, 1981·Cell·R A Laskey, R M Harland
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D J BergsmaK N Subramanian
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Jan 1, 1984·Annual Review of Biochemistry·G R Stark, G M Wahl
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·E H BlackburnT Ryan
Sep 1, 1984·Cell·R H Reeder
Jan 1, 1980·Annual Review of Genetics·M A Gorovsky
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Y M Sanford, E Orias
Jul 24, 1981·Nucleic Acids Research·T R Cech, S L Brehm

❮ Previous
Next ❯

Citations

Oct 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R M BenbowD D Larson
Jan 1, 1992·Developmental Genetics·C F Brunk, P A Navas
Jan 1, 1995·Developmental Genetics·A La TerzaP Luporini
Aug 1, 1988·Molecular & General Genetics : MGG·M E Silliker, O R Collins
Dec 26, 2001·Trends in Genetics : TIG·Aaron P TurkewitzGeoffrey Kapler
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G L YuE H Blackburn
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A LøvlieE Orias
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G L Yu, E H Blackburn
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Gaertig, M A Gorovsky
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R W KahnC F Brunk
Jul 1, 1991·The Journal of protozoology·E OriasT Higashinakagawa
May 1, 1991·The Journal of protozoology·D D LarsonW L Shaiu
Mar 1, 1991·The Journal of protozoology·S E Baird, L A Klobutcher
Jan 1, 1995·The Journal of Eukaryotic Microbiology·D P Romero, E H Blackburn
Jun 11, 1999·The Journal of Eukaryotic Microbiology·M ErbeznikC L Jahn
Aug 13, 2003·Eukaryotic Cell·Jeffrey D Wuitschick, Kathleen M Karrer
Jun 21, 1991·European Journal of Protistology·B Kunze, G Cleffmann
Jul 31, 1987·Cell·D D LarsonE H Blackburn
Jan 21, 2009·The EMBO Journal·Taraka R DontiGeoffrey M Kapler
Dec 1, 2004·Annual Review of Genetics·John Tower

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
M M Tondravi, M C Yao
Proceedings of the National Academy of Sciences of the United States of America
A LøvlieE Orias
Proceedings of the National Academy of Sciences of the United States of America
P J BrunsE H Blackburn
© 2021 Meta ULC. All rights reserved