PMID: 6161368Nov 1, 1980Paper

Control of serum protein production in hepatocyte hybridomas: immortalization and expression of normal hepatocyte genes

Proceedings of the National Academy of Sciences of the United States of America
J SzpirerJ C Wanson

Abstract

"Hepatocyte hybridomas" have been isolated after fusion of adult hepatocytes and alpha-fetoprotein (AFP)-producing mouse hepatoma cells. The yield of viable hybrid clones was low but could be increased by culturing the cells in the presence of insulin. On the basis of their chromosome constitution, the hybrids were classified into two groups characterized by either a single or a double set of mouse (hepatoma) chromosomes. The hybrids segregated rat chromosomes and thus constitute an excellent material for gene mapping studies in the rat. Most of the hepatocyte hybridomas retained the production of one or more rat serum proteins, indicating that the corresponding structural genes, contributed by the normal hepatocyte parent, have been immortalized and maintained in the active state after fusion. However, these hybrids do not produce rat AFP, although mouse AFP synthesis is maintained. This result strongly suggests that silent rat (hepatocyte) AFP genes coexist in hepatocyte hybridoma nuclei with active mouse (hepatoma) AFP genes. Finally, on the basis of certain properties of these hepatocyte-hepatoma hybrids, we suggest that the nondividing state of the hepatocytes is actively controlled by a regulatory mechanism which prevents...Continue Reading

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Citations

Jan 1, 1985·Immunogenetics·C PerlmannG Klein
Sep 1, 1988·Somatic Cell and Molecular Genetics·B DahlbäckG Levan
May 1, 1982·Somatic Cell Genetics·G J DarlingtonG Schlanger
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D PlatikaM C Fishman
Sep 1, 1983·The Journal of Cell Biology·H Baumann, G P Jahreis
Jul 1, 1984·Experimental Cell Research·S M JudgeS Liao
May 1, 1989·Molecular and Cellular Biology·H GourdeauR E Fournier
Feb 1, 1990·Molecular and Cellular Biology·R A RippeH L Leffert

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