PMID: 4390641Jul 1, 1969

Control of specific gene expression examined in synchronized mammalian cells

Proceedings of the National Academy of Sciences of the United States of America
D MartinM A Bresler

Abstract

The control of synthesis of the steroid-inducible enzyme, tyrosine aminotransferase (TAT), has been studied in synchronized cultures of HTC cells, an established line of rat hepatoma cells. During the mitotic and early G1 periods of the generation cycle of cells previously exposed to inducer, TAT synthesis is maximal in the absence of inducer, i.e., synthesis is constitutive. (By contrast, in random cells maximal TAT synthesis is always dependent upon the presence of the inducer.) After the third hour of G1 "G1[3]"), TAT synthesis is inhibited by a specific posttranscriptional repressor, the formation of which requires RNA synthesis. We speculate that this repressor is antagonized by the steroid inducer. The degradation of TAT during mitosis and G1 is constant and not affected by cycloheximide or actinomycin D in our experiments.

References

Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·G M TomkinsB Peterkofsky
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·E B ThompsonJ F Curran
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Sep 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·J R Reel, F T Kenney
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Citations

Jan 1, 1971·In Vitro·G F Whitmore
Jan 1, 1984·The International Journal of Biochemistry·J V GroenewaldW Oelofsen
Mar 18, 1976·The New England Journal of Medicine·T D Gelehrter
Oct 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·T D Gelehrter, G M Tomkins
Apr 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·D Martin, G M Tomkins
Jun 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·T D Gelehrter, G M Tomkins
Oct 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·E B Thompson, T D Gelehrter
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J N LoebL L Yeung
Oct 1, 1970·The Journal of Cell Biology·P L Ballard, G M Tomkins
Jul 22, 2004·Annals of the New York Academy of Sciences·Naima Ismaili, Michael J Garabedian
Aug 28, 1970·Journal of Molecular Biology·H H Samuels, G M Tomkins
Dec 14, 1970·Journal of Molecular Biology·E B ThompsonG M Tomkins
Feb 1, 1974·Metabolism: Clinical and Experimental·E B Thompson, M E Lippman
Jan 1, 1973·Metabolism: Clinical and Experimental·T D Gelehrter
Apr 1, 1980·Journal of Neurochemistry·M R Pishak, A T Phillips
Jul 1, 1974·Archives of Biochemistry and Biophysics·Y Sato, M Maruyama
Apr 21, 1975·Biochemical and Biophysical Research Communications·J R Emanuel, T D Gelehrter
Aug 1, 1971·Journal of Cellular Physiology·F Grinnell, P A Shere
Apr 1, 1975·Journal of Cellular Physiology·R D IvarieG M Tomkins
Jan 26, 1972·Nature: New Biology·S R Levisohn, E B Thompson

Related Concepts

Metazoa
Actidione
Ac-De
Oradexon
Enzyme Induction
Enzyme Repression
Genes, Regulator
Genetic Code
Liver Carcinoma
Malignant Neoplasm of Liver

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