Control of TCF-4 expression by VDR and vitamin D in the mouse mammary gland and colorectal cancer cell lines.

PloS One
Marcy E BeildeckStephen W Byers

Abstract

The vitamin D receptor (VDR) pathway is important in the prevention and potentially in the treatment of many cancers. One important mechanism of VDR action is related to its interaction with the Wnt/beta-catenin pathway. Agonist-bound VDR inhibits the oncogenic Wnt/beta-catenin/TCF pathway by interacting directly with beta-catenin and in some cells by increasing cadherin expression which, in turn, recruits beta-catenin to the membrane. Here we identify TCF-4, a transcriptional regulator and beta-catenin binding partner as an indirect target of the VDR pathway. In this work, we show that TCF-4 (gene name TCF7L2) is decreased in the mammary gland of the VDR knockout mouse as compared to the wild-type mouse. Furthermore, we show 1,25(OH)2D3 increases TCF-4 at the RNA and protein levels in several human colorectal cancer cell lines, the effect of which is completely dependent on the VDR. In silico analysis of the human and mouse TCF7L2 promoters identified several putative VDR binding elements. Although TCF7L2 promoter reporters responded to exogenous VDR, and 1,25(OH)2D3, mutation analysis and chromatin immunoprecipitation assays, showed that the increase in TCF7L2 did not require recruitment of the VDR to the identified elements ...Continue Reading

References

Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H M Darwish, H F DeLuca
Aug 15, 1980·Science·A W NormanG M Grodsky
Jul 2, 1998·Biochemical and Biophysical Research Communications·M AlonsoR Perez-Fernandez
Sep 4, 1998·Science·T C HeK W Kinzler
Mar 11, 1999·Current Opinion in Genetics & Development·P Polakis
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·M ShtutmanA Ben-Ze'ev
Dec 23, 1999·Current Biology : CB·V EaswaranS Byers
Nov 1, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N Barker, H Clevers
Jan 4, 2001·Cell·T A GrahamW Xu
Mar 27, 2001·Nucleic Acids Research·H BrantjesH Clevers
Feb 22, 2002·The Journal of Biological Chemistry·David J MulhollandColleen C Nelson
May 10, 2002·The Journal of Biological Chemistry·Salimuddin ShahStephen W Byers
Dec 6, 2002·Oncogene·Dennis R Chesire, William B Isaacs
Mar 20, 2003·Molecular and Cellular Endocrinology·Glendon M ZinserJoEllen Welsh
Jun 12, 2003·The Journal of Biological Chemistry·Avital L AmirSteven P Balk
Mar 3, 2004·Oncogene·Karen RotherKurt Engeland
Jul 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Glendon M Zinser, JoEllen Welsh
Jan 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Emmelie A JanssonSven Pettersson
Apr 5, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Makoto SaegusaIsao Okayasu
Jun 23, 2005·Diabetologia·C MathieuR Bouillon
Mar 18, 2006·Molecular Cell·Salimuddin ShahStephen W Byers
Jan 16, 2007·The Journal of Steroid Biochemistry and Molecular Biology·J Wesley PikeNirupama K Shevde
Aug 21, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Wu ZhangHeinz-Josef Lenz
Aug 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jan WehkampEduard F Stange

❮ Previous
Next ❯

Citations

Oct 19, 2011·Future Medicinal Chemistry·Michael Kahn
Jul 20, 2010·Current Opinion in Gastroenterology·Jun Sun
Jun 9, 2012·Breast Cancer Research : BCR·Nair LopesFernando Schmitt
Oct 3, 2013·Nutrients·Meis Moukayed, William B Grant
Mar 10, 2011·The Proceedings of the Nutrition Society·Elizabeth K LundIan T Johnson
Dec 20, 2011·Carcinogenesis·Charles GiardinaDaniel W Rosenberg
Mar 1, 2012·Current Colorectal Cancer Reports·Rong LuJun Sun
Feb 12, 2011·Expert Review of Gastroenterology & Hepatology·Cindy D Davis, John A Milner
Oct 16, 2013·Journal of Cardiac Failure·Anna J Meredith, Bruce M McManus
Aug 15, 2013·Critical Reviews in Oncology/hematology·Michelino Di RosaLucia Malaguarnera
Aug 4, 2015·Chinese Journal of Natural Medicines·Rachel A NessWei Li
Dec 16, 2011·The Biochemical Journal·James C FleetYan Li
Apr 10, 2015·Pharmaceutical Biology·Marco GiammancoGaetano Leto
Sep 2, 2018·Diabetes/metabolism Research and Reviews·Miji KimJesse Roth
Nov 10, 2013·Cancers·María Jesús LarribaAlberto Muñoz
Aug 29, 2020·Cancers·Asunción Fernández-BarralAlberto Muñoz
Jan 20, 2012·Molecular Cancer Research : MCR·Jing-Xue WangXue-Tao Pei
Dec 17, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Zhiwei LiKeping Xie
Nov 25, 2020·Cancers·José Manuel González-SanchoAlberto Muñoz

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear translocation
immunoprecipitation
pull-down
pulled down
transfection
PCR
ChIP
scraping
Assay
Pulldown

Software Mentioned

Prism
GraphPad
TopFlash

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.