PMID: 11927669Apr 3, 2002Paper

Control of the propagation of dendritic low-threshold Ca(2+) spikes in Purkinje cells from rat cerebellar slice cultures

The Journal of Physiology
Pauline CavelierJean-Louis Bossu

Abstract

To investigate the ionic mechanisms controlling the dendrosomatic propagation of low-threshold Ca(2+) spikes (LTS) in Purkinje cells (PCs), somatically evoked discharges of action potentials (APs) were recorded under current-clamp conditions. The whole-cell configuration of the patch-clamp method was used in PCs from rat cerebellar slice cultures. Full blockade of the P/Q-type Ca(2+) current revealed slow but transient depolarizations associated with bursts of fast Na(+) APs. These can occur as a single isolated event at the onset of current injection, or repetitively (i.e. a slow complex burst). The initial transient depolarization was identified as an LTS Blockade of P/Q-type Ca(2+) channels increased the likelihood of recording Ca(2+) spikes at the soma by promoting dendrosomatic propagation. Slow rhythmic depolarizations shared several properties with the LTS (kinetics, activation/inactivation, calcium dependency and dendritic origin), suggesting that they correspond to repetitively activated dendritic LTS, which reach the soma when P/Q channels are blocked. Somatic LTS and slow complex burst activity were also induced by K(+) channel blockers such as TEA (2.5 x 10(-4) M) charybdotoxin (CTX, 10(-5) M), rIberiotoxin (10(-7) ...Continue Reading

References

Aug 1, 1988·The Journal of Physiology·J Hounsgaard, J Midtgaard
Mar 1, 1989·Pflügers Archiv : European journal of physiology·T Hirano, S Hagiwara
Dec 1, 1981·Journal of Neuroscience Methods·B H Gähwiler
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·J EilersA Konnerth
Jan 1, 1994·Journal of Neurophysiology·E De Schutter, J M Bower
Jan 1, 1996·Annual Review of Physiology·W E Crill
Jan 1, 1996·Annual Review of Neuroscience·D JohnstonB R Cristie
Mar 1, 1997·Trends in Neurosciences·G StuartM Häusser
Jun 12, 1997·Nature·S L HoffmanM J Morgan
Mar 3, 1999·The European Journal of Neuroscience·T D Jacquin, D L Gruol
Jul 22, 1999·Annals of the New York Academy of Sciences·W A CoetzeeB Rudy
Nov 7, 1999·Progress in Brain Research·A DestexheT J Sejnowski

❮ Previous
Next ❯

Citations

Sep 3, 2008·Pflügers Archiv : European journal of physiology·Narimane Benhassine, Thomas Berger
Dec 8, 2004·Progress in Histochemistry and Cytochemistry·Josef P Kapfhammer
Sep 16, 2016·Frontiers in Cellular Neuroscience·Tobias Bock, Greg J Stuart
Jul 1, 2009·Therapeutic Advances in Neurological Disorders·Michael Strupp, Thomas Brandt
Jul 18, 2017·Expert Opinion on Drug Discovery·Frédéric DoussauJean Louis Bossu
Feb 28, 2008·Neuroreport·Pauline CavelierJean-Louis Bossu
Jul 9, 2005·The Journal of Physiology·Bruce E McKay, Ray W Turner
Oct 8, 2005·The Journal of Physiology·Simin KhavandgarKamran Khodakhah
Jun 17, 2009·Expert Opinion on Pharmacotherapy·Thomas BrandtMichael Strupp
Jul 3, 2009·Journal of Neurophysiology·Kirsten ThomsenMartin Lauritzen
Dec 23, 2003·The European Journal of Neuroscience·Fivos VogalisBarrie Lancaster

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.