Control of the timing of promoter escape and RNA catalysis by the transcription factor IIb fingertip.

The Journal of Biological Chemistry
Khiem Tran, Jay D Gralla

Abstract

Transcription factor IIB (TFIIB) recruits RNA polymerase II to promoters and inserts a finger domain into its active site, with unknown consequences. Here we show that that the tip of this finger is important for two transcription initiation functions. First, TFIIB acts as a catalytic cofactor for initial RNA bond formation. It does so via a pair of fingertip aspartates that can bind magnesium, placing TFIIB within a family of proteins that insert finger domains to alter the catalytic functions of RNA polymerase. Second, the TFIIB fingertip mediates the timing of the release of TFIIB that is associated with appropriate promoter escape. These initiation requirements may assist in RNA quality control by minimizing functional synthesis when RNA polymerase becomes inappropriately associated with the genome without having been recruited there by TFIIB.

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Citations

Jul 11, 2009·The Journal of Biological Chemistry·Nancy E ThompsonRichard R Burgess
Oct 1, 2010·The Journal of Biological Chemistry·Khiem Tran, Jay D Gralla
Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Pavel ČabartDonal S Luse
Oct 26, 2011·Molecular and Cellular Biology·James Fishburn, Steven Hahn
Sep 18, 2012·Biochimica Et Biophysica Acta·Donal S Luse
Sep 25, 2012·Biochimica Et Biophysica Acta·Xin LiuRoger D Kornberg
Sep 17, 2011·Science·Srivatsava NaiduJoost C B M Zomerdijk
Jan 25, 2012·International Journal of Molecular Sciences·Shahram GolbabapourMaryam Hajrezaei
Apr 18, 2018·Protein Science : a Publication of the Protein Society·Jenna K Rimel, Dylan J Taatjes
Aug 14, 2018·Nucleic Acids Research·Stefan DexlMichael Thomm

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