Control of the Yeast Mating Pathway by Reconstitution of Functional α-Factor Using Split Intein-Catalyzed Reactions

ACS Synthetic Biology
Ka-Hei Siu, Wilfred Chen

Abstract

Synthetic control strategies using signaling peptides to regulate and coordinate cellular behaviors in multicellular organisms and synthetic consortia remain largely underdeveloped because of the complexities necessitated by heterologous peptide expression. Using recombinant proteins that exploit split intein-mediated reactions, we presented here a new strategy for reconstituting functional signaling peptides capable of eliciting desired cellular responses in S. cerevisiae. These designs can potentially be tailored to any signaling peptides to be reconstituted, as the split inteins are promiscuous and both the peptides and the reactions are amenable to changes by directed evolution and other protein engineering tools, thereby offering a general strategy to implement synthetic control strategies in a large variety of applications.

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Citations

Mar 11, 2020·Annual Review of Chemical and Biomolecular Engineering·Emily A BerckmanWilfred Chen
Nov 2, 2019·Nature Communications·Navaneethan PalanisamyBarbara Di Ventura
Nov 2, 2019·Nucleic Acids Research·Femi J OlorunnijiSusan J Rosser

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