Abstract
Using pSC101, RSF1010, RSF2124 and RP4 plasmids as vectors and bacteriophage lambdatrpD-A60-3 DNA as a source of the Escherichia coli whole tryptophan operon, composite plasmids of pSC101-trp, RSF1010-trp, RSF2124-trp and RP4-trp were constructed in vitro with EcoRI restriction endonuclease and DNA ligase. Each composite plasmid could be maintained stably in E. coli cells. The copy number of pSC101-trp, RSF1010-trp, RSF2124-trp and RP4-trp were 4.2, 11.2, 11.9 and 1.6 per chromosome respectively. The tryptophan synthetase activities in cells containing pSC101-trp, RSF1010-trp, RSF2124-trp aand RP4-trp plasmid were found to be 2.1, 6.0, 5.0 and 2.5 times compared with the level in chromosomal trp+ cells when they were grown in a minimal medium. By partial derepression with indolylacrylic acid, the enzyme levels were elevated to 10.1, 16.3, 15.3, 12.3 times, respectively, that of the control cells. The tryptophan synthetase activities did not increase in proportion to the copy number of the plasmids, but were strongly affected by the repression system of host cells.
References
May 20, 1975·Biochemistry·T TanakaR Inman
Dec 30, 1975·Molecular & General Genetics : MGG·M SoS Falkow
Jun 12, 1975·Nature·L H KedesS N Cohen
Oct 1, 1975·Cell·A C ChangS N Cohen
Feb 25, 1975·Journal of Molecular Biology·T Yamamoto, F Imamoto
Sep 1, 1974·Virology·M Fiandt, W Szybalski
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S N CohenR B Helling
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J F MorrowR B Helling
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·V HershfieldD R Helinski
Oct 27, 1970·Biochemistry·D B Clewell, D R Helinski
Oct 26, 1971·European Journal of Biochemistry·M SchachnerW Zillig
Citations
Jan 17, 1978·Molecular & General Genetics : MGG·K Nagahari, K Sakaguchi
Aug 1, 1979·Molecular & General Genetics : MGG·P CossartG N Cohen
Jun 20, 1979·Molecular & General Genetics : MGG·L J Wallace, G Wilcox
Mar 20, 1979·Molecular & General Genetics : MGG·K NagahariK Sakaguchi
Jan 1, 1980·Molecular & General Genetics : MGG·F Gautier, R Bonewald
Jan 1, 1981·Molecular & General Genetics : MGG·J ChenaisJ C Patte
Dec 1, 1986·Analytical Biochemistry·M A Hediger
Aug 14, 1984·Biochimica Et Biophysica Acta·T SekiguchiY Nosoh
Mar 1, 1990·FEMS Microbiology Letters·O Rivero-LezcanoG Naharro-Carrasco
Jul 1, 1980·Gene·K NagahariK Sakaguchi
Mar 1, 1993·Bio/technology·D MurdockM Thalen
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·Y MachidaJ E Ikeda
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M A HedigerI Zabin
Dec 1, 1985·Biotechnology and Bioengineering·S B LeeJ E Bailey
May 1, 1984·Biotechnology and Bioengineering·S H Kim, D D Ryu
Dec 31, 1985·Biochemical and Biophysical Research Communications·G del RealJ F Martín
Apr 21, 1977·Nature·K NagahariK Sakaguchi
Jun 1, 1991·FEMS Microbiology Letters·O RiveroG Naharro
Jan 1, 1981·Annals of the New York Academy of Sciences·T Imanaka, S Aiba
Dec 21, 2016·Microbial Cell Factories·Michael JahnSusann Müller
Oct 23, 2019·Journal of Biotechnology·Julia TröndleDirk Weuster-Botz
May 9, 1998·Infection and Immunity·C Hernanz MoralG Naharro Carrasco
May 1, 1981·Journal of Bacteriology·P E Berg
Oct 1, 1988·Journal of Bacteriology·M Matsuoka, B A McFadden
Mar 1, 1978·Journal of Bacteriology·K Nagahari, K Sakaguchi
Mar 1, 1992·Applied and Environmental Microbiology·J AnguitaG Naharro
Jul 1, 1978·Journal of Bacteriology·M Mergeay, J Gerits
Aug 1, 1993·Applied and Environmental Microbiology·J AnguitaG Naharro
Sep 1, 1979·Journal of Bacteriology·T Tanaka
Dec 1, 1994·Journal of Bacteriology·L M MateosJ F Martín
Jul 1, 1990·Journal of Bacteriology·O RiveroG Naharro
Mar 1, 1979·Microbiological Reviews·B W HollowayA F Morgan
Feb 1, 1982·Applied and Environmental Microbiology·S AibaT Imanaka