Control of vitellogenin genes expression by sequences derived from transposable elements in rainbow trout

Biochimica Et Biophysica Acta
Anthony BouterOlivier Sire

Abstract

In most of oviparous animals, vitellogenins (VTG) are the major egg yolk precursors. They are produced in the liver under the control of estrogens. In rainbow trout (Oncorhynchus mykiss), the vtg genes cluster contains an unusually large number of almost identical gene copies. In order to identify the regulatory elements in their promoters, we used a combination of reporter plasmids containing genomic sequences including putative estrogen response elements (EREs) and we performed transient transfection assays in MCF-7 and yeast cells. We found a functional ERE corresponding to the sequence GGGGCAnnnTAACCT (rtvtgERE), which differs from the consensus ERE (ERE(cs)) by three base pairs. This non-palindromic ERE is located in the env gene of a retrotransposon relic, 180 base pairs upstream of the transcriptional start site. Fluorescence anisotropy experiments confirmed that the purified human estrogen receptor alpha (hERalpha) can specifically bind to rtvtgERE. Furthermore, we observe that the stability of hERalpha-ERE(cs) and hERalpha-rtvtgERE complexes is similar with equilibrium dissociation constants of 3.0nM and 6.2nM respectively, under our experimental conditions. Additionally, this rtvtgERE sequence displays a high E2-respo...Continue Reading

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Citations

Jun 6, 2013·International Journal of Endocrinology·Gwenneg KerdivelFarzad Pakdel
Oct 21, 2014·International Journal of Molecular Sciences·Hee Jeong KongCheul Min An
Oct 30, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Ursula A BussmannJ Lino Barañao
Jan 18, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Adélaïde Le GrandVéronique Le Tilly
Jun 2, 2020·International Journal of Environmental Health Research·Manel ChebbiVéronique Le Tilly

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