Control of Wnt/β-Catenin Signaling Pathway in Vivo via Light Responsive Capsules

ACS Nano
Alfredo AmbrosoneClaudia Tortiglione

Abstract

The possibility to remotely manipulate intracellular pathways in single cells is among the current goals of biomedicine, demanding new strategies to control cell function and reprogramming cell fate upon external triggering. Optogenetics is one approach in this direction, allowing specific cell stimulation by external illumination. Here, we developed optical switchers of an ancient and highly conserved system controlling a variety of developmental and adult processes in all metazoans, from Hydra to mammals, the Wnt/β-catenin signaling pathway. An intracellular modulator of the Wnt pathway was enclosed into polyelectrolyte multilayer microcapsules engineered to include self-tracking (i.e., fluorescence labeling) and light mediated heating functionalities (i.e., plasmonic nanoparticles). Capsules were delivered in vivo to Hydra and NIR triggered drug release caused forced activation of the Wnt pathway. The possibility to remotely manipulate the Wnt pathway by optical switchers may be broadly translated to achieve spatiotemporal control of cell fate for new therapeutic strategies.

References

Sep 24, 2005·Nano Letters·Andre G SkirtachGleb B Sukhorukov
Jun 23, 2006·Angewandte Chemie·Andre G SkirtachGleb B Sukhorukov
Oct 11, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·A Muñoz JavierW J Parak
Feb 4, 2009·Trends in Biotechnology·Jingsong ZhaoArie Abo
Feb 17, 2009·Developmental Biology·Tobias LengfeldThomas W Holstein
Jul 22, 2009·Developmental Cell·Bryan T MacDonaldXi He
Sep 9, 2009·Development·Renée van Amerongen, Roel Nusse
Apr 30, 2010·Science Translational Medicine·Steven MinearJill A Helms
Jul 21, 2010·Nanoscale·Loretta L del MercatoWolfgang J Parak
Oct 30, 2010·Nature Methods·Björn C Knollmann
May 19, 2011·Macromolecular Rapid Communications·Weijun TongHelmuth Möhwald
Jan 10, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Susana Carregal-RomeroWolfgang J Parak
Jun 23, 2012·Cold Spring Harbor Perspectives in Biology·Jemima L WhyteJill A Helms
Jul 4, 2012·Cold Spring Harbor Perspectives in Biology·Thomas W Holstein
Aug 14, 2012·Transcription·Steve GerondakisRaelene J Grumont
Sep 7, 2012·Cold Spring Harbor Perspectives in Biology·Bradley J Merrill
Sep 12, 2012·Nature Biotechnology·Arie Abo, Hans Clevers
Dec 1, 2012·Toxicology Mechanisms and Methods·Alfredo Ambrosone, Claudia Tortiglione
Dec 22, 2012·Nature Reviews. Cancer·Jamie N Anastas, Randall T Moon
Mar 2, 2013·ACS Nano·Valentina MarchesanoClaudia Tortiglione
Oct 18, 2014·Frontiers in Bioengineering and Biotechnology·Alfredo AmbrosoneAngela Tino
Mar 15, 2015·Science·Ritchie ChenPolina Anikeeva
Aug 13, 2015·Nanomaterials·Valentina MarchesanoSilvia Giordani

❮ Previous
Next ❯

Citations

Feb 1, 2017·Science Advances·Claudia TortiglioneGuglielmo Lanzani
Jan 18, 2018·Antioxidants & Redox Signaling·Shamim Akhtar SufiRukkumani Rajagopalan
Nov 28, 2017·Advanced Functional Materials·Xiuying LiZhenpeng Qin
Jun 15, 2019·Advanced Materials·Jingchao LiKanyi Pu
Jun 14, 2019·Frontiers in Bioengineering and Biotechnology·Ilaria SanzariAlfredo Ambrosone
Dec 29, 2016·Polymers·Valentina MarturanoVeronica Ambrogi
Aug 19, 2020·Nature Materials·Guillaume BaffouRomain Quidant
May 22, 2019·Polymers·Domenico PironeBartosz Tylkowski
Apr 14, 2020·IScience·Maria MorosClaudia Tortiglione
Jan 4, 2017·Bioconjugate Chemistry·Mikhail V ZyuzinWolfgang J Parak
Mar 16, 2017·ACS Nano·Beatriz PelazWolfgang J Parak
Mar 7, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Mikhail V ZyuzinGleb B Sukhorukov
Apr 10, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Bogdan V ParakhonskiyAndre G Skirtach
Sep 10, 2019·ACS Applied Materials & Interfaces·Giulia VeronesiClaudia Tortiglione
Dec 12, 2018·ACS Applied Materials & Interfaces·Maria MorosAntonios G Kanaras
Apr 14, 2018·Bioconjugate Chemistry·Adrián Jiménez-BalsaLino Ferreira
Jun 28, 2019·ACS Chemical Neuroscience·Mohammed Nadim SardoiwalaSubhasree Roy Choudhury

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.

Related Papers

Journal of Cell Science
Joerg Huelsken, Juergen Behrens
Journal of Cell Science
J Hlsken, J Behrens
Nature Methods
Peter Karagiannis, Shinya Yamanaka
Expert Opinion on Drug Delivery
Bruno G De GeestHelmuth Möhwald
© 2022 Meta ULC. All rights reserved